| Autor: |
Gong TY; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China Graduate School, Medical School of Chinese PLA, Beijing 100039, China., Guo L; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China., Ye K; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China., Zhao Q; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China., Ye LY; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China., Ma YN; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China., Wang LF; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China., Yang JY; Department of Laboratory Medicine, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China. |
| Abstrakt: |
Objective: To analyze the phenotypic and genotypic characteristics of Escherichia coli causing bloodstream and abdominal co-infection (CoECO), and provide clues for empirical antibiotics treatment. Methods: The strains of Escherichia coli isolated from blood and abdominal samples in the Department of Laboratory Medicine of the First Medical Center of the PLA General Hospital from 2010 to 2020 were retrospectively analyzed. Mass spectrometer was used to identify all of the strains and the minimum inhibitory concentration (MIC) were detected by VITEK 2 Compact. All isolates were sequenced by 2×150 bp double terminal sequencing strategy on the HiSeq X Ten sequencer (Illumina). After the genome sequence was spliced, the single nucleotide polymorphism (SNP) analysis of the strain sequence was performed using kSNP3 software to clarify the homologous relationship between strains. If the strains isolated from two different parts had high homology, they were regarded as the same strain and the case was with CoECO infection. Meanwhile, the multilocus sequence type (MLST) was determined using PubMLST website and resistant genes were screened by CARD website. Results: A total of 70 cases of CoECO infection were screened, including 45 males and 25 females, and aged (59.2±16.3) years old. The 70 CoECO isolates belonged to 35 sequence types (STs). The most prevalent STs included ST38 ( n =6), ST 405 ( n =6), ST 1193 ( n =6) and ST131 ( n =5), and other ST types contained less than 5 strains. The homologous relationship among strains was relatively scattered, presenting a sporadic trend as a whole, and only a few strains had a small-scale outbreak. The CoECO isolates showed significantly resistance to ampicillin (91.4%, 64/70), ampicillin/sulbactam (74.3%, 5 2/70), ceftriaxone (72.9%, 51/70), ciprofloxacin (71.4%, 50/70) and levofloxacin (71.4%, 50/70), and high-sensitivity to piperacillin/tazobactam, carbapenems and amikacin. The most prevalent resistant gene was tet (A/B) (70%, 49/70), followed by bla TEM (58.6%, 41/70), sul1 (55.7%, 40/70), sul2 (54.3%, 38/70), bla CTX-M-14 (25.7%, 18/70), bla CTX-M-15 (17.1%, 13/70), bla CTX-M-55 (15.7%, 11/70), bla CTX-M-64/65 (5.7%, 4/70), bla CTX-M-27 (4.3%, 3/70), mcr-1 (4.3%, 3/70), bla NDM-5 (2.9%, 2/70). Conclusions: CoECO is distributed dispersedly and has no obvious advantage clone. No genotype with obvious advantages was found. Although the strain has a high resistance rate to some antibacterial drugs, the proportion of carrying resistant genes is low, and it has a high sensitivity to some first-line antibacterial drugs. |
