How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells.

Autor: Farlow A; Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Campus Botucatu, 18618-691, Botucatu, SP, Brazil., José Crevelin E; Department of Chemistry, School of Philosophy, Sciences and Languages, University of São Paulo (USP), 14040-901, Ribeirão Preto, SP, Brazil., Basso Santiago K; Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Campus Botucatu, 18618-691, Botucatu, SP, Brazil., de Oliveira Cardoso E; Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Campus Botucatu, 18618-691, Botucatu, SP, Brazil., Matsumoto da Silva L; Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Campus Botucatu, 18618-691, Botucatu, SP, Brazil., Kenupp Bastos J; Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, University of São Paulo (USP), 14040-903, Ribeirão Preto, SP, Brazil., Maurício Sforcin J; Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Campus Botucatu, 18618-691, Botucatu, SP, Brazil.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2023 May; Vol. 20 (5), pp. e202300348. Date of Electronic Publication: 2023 Apr 07.
DOI: 10.1002/cbdv.202300348
Abstrakt: The aim of this study was to identify propolis compounds after incubation of normal and tumor cells (monocytes and HEp-2 cells, respectively) with Brazilian green propolis, in the lysate and supernatant of cell cultures and within these cells by gas chromatography-mass spectrometry (GC/MS). Cinnamic acid derivatives were generally localized in the lysate of both cell lines after incubation, suggesting these compounds are actively transported across the membrane into the cytoplasm. Terpenes were also found in the lysate. Artepillin C, in contrast, was localised only in the supernatant. Some constituents were unobservable after incubation, especially in monocytes, suggesting the compounds had been degraded. Our findings shed light on the possible sites of action (intracellular or via a cell membrane protein) and the bioavailability of various constituents of propolis, as well as possible modes of delivery of bioactive constituents.
(© 2023 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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