Development of pH-Sensitive Magnetoliposomes Containing Shape Anisotropic Nanoparticles for Potential Application in Combined Cancer Therapy.

Autor: Pacheco ARF; Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.; Associate Laboratory LaPMET, 4710-057 Braga, Portugal., Cardoso BD; Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.; Associate Laboratory LaPMET, 4710-057 Braga, Portugal., Pires A; Associate Laboratory LaPMET, 4169-007 Porto, Portugal.; IFIMUP-Instituto de Física dos Materiais, University of Porto, R. Campo Alegre, 4169-007 Porto, Portugal., Pereira AM; Associate Laboratory LaPMET, 4169-007 Porto, Portugal.; IFIMUP-Instituto de Física dos Materiais, University of Porto, R. Campo Alegre, 4169-007 Porto, Portugal., Araújo JP; Associate Laboratory LaPMET, 4169-007 Porto, Portugal.; IFIMUP-Instituto de Física dos Materiais, University of Porto, R. Campo Alegre, 4169-007 Porto, Portugal., Carvalho VM; MEtRICs, Mechanical Engineering Department, University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal.; ALGORITMI Center, University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal.; Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal., Rodrigues RO; Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal., Coutinho PJG; Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.; Associate Laboratory LaPMET, 4710-057 Braga, Portugal., Castelo-Grande T; LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal., Augusto PA; LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal., Barbosa D; LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal., Lima RA; MEtRICs, Mechanical Engineering Department, University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal.; CEFT-Transport Phenomena Research Center, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal., Teixeira SFCF; ALGORITMI Center, University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal., Rodrigues ARO; Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.; Associate Laboratory LaPMET, 4710-057 Braga, Portugal., Castanheira EMS; Physics Centre of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.; Associate Laboratory LaPMET, 4710-057 Braga, Portugal.
Jazyk: angličtina
Zdroj: Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2023 Mar 15; Vol. 13 (6). Date of Electronic Publication: 2023 Mar 15.
DOI: 10.3390/nano13061051
Abstrakt: Late diagnosis and systemic toxicity associated with conventional treatments make oncological therapy significantly difficult. In this context, nanomedicine emerges as a new approach in the prevention, diagnosis and treatment of cancer. In this work, pH-sensitive solid magnetoliposomes (SMLs) were developed for controlled release of the chemotherapeutic drug doxorubicin (DOX). Shape anisotropic magnetic nanoparticles of magnesium ferrite with partial substitution by calcium (Mg 0.75 Ca 0.25 Fe 2 O 4 ) were synthesized, with and without calcination, and their structural, morphological and magnetic properties were investigated. Their superparamagnetic properties were evaluated and heating capabilities proven, either by exposure to an alternating magnetic field (AMF) (magnetic hyperthermia) or by irradiation with near-infrared (NIR) light (photothermia). The Mg 0.75 Ca 0.25 Fe 2 O 4 calcined nanoparticles were selected to integrate the SMLs, surrounded by a lipid bilayer of DOPE:Ch:CHEMS (45:45:10). DOX was encapsulated in the nanosystems with an efficiency above 98%. DOX release assays showed a much more efficient release of the drug at pH = 5 compared to the release kinetics at physiological pH. By subjecting tumor cells to DOX-loaded SMLs, cell viability was significantly reduced, confirming that they can release the encapsulated drug. These results point to the development of efficient pH-sensitive nanocarriers, suitable for a synergistic action in cancer therapy with magnetic targeting, stimulus-controlled drug delivery and dual hyperthermia (magnetic and plasmonic) therapy.
Databáze: MEDLINE
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