| Autor: |
Souza-Gonçalves J; Instituto Superior de Agronomia, Universidade de Lisboa, LEAF-Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, 1349-017 Lisbon, Portugal.; Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal., Fialho A; Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisbon, Portugal.; iBB-Institute for Bioengineering and Biosciences and i4HB-Institute for Health and Bioeconomy, Instituto Superior Técnico, 1049-001 Lisbon, Portugal., Soares CMF; Institute of Technology and Research (ITP), Avenida Murilo Dantas 300-Farolandia, Aracaju 49032-490, Brazil.; Tiradentes University (UNIT), Avenida Murilo Dantas 300-Farolandia, Aracaju 49032-490, Brazil., Osório NM; Instituto Politécnico de Setúbal, Escola Superior de Tecnologia do Barreiro, 2839-001 Lavradio, Portugal.; Instituto Superior de Agronomia, Universidade de Lisboa, Centro de Estudos Florestais, Associated Laboratory TERRA, 1349-017 Lisbon, Portugal., Ferreira-Dias S; Instituto Superior de Agronomia, Universidade de Lisboa, LEAF-Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, 1349-017 Lisbon, Portugal.; Instituto Superior de Agronomia, Universidade de Lisboa, Laboratório de Estudos Técnicos, 1349-017 Lisbon, Portugal. |
| Abstrakt: |
Crude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, low-calorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with medium-chain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn -2 and medium-chain fatty acids (M) at positions sn -1,3 (MLM). Crude OPO exhibited a high acidity (12.05-28.75% free fatty acids), and high contents of chlorophylls and oxidation products. Reactions were carried out continuously in a packed-bed bioreactor for 70 h, using sn -1,3 regioselective commercial immobilized lipases ( Thermomyces lanuginosus lipase, Lipozyme TL IM; and Rhizomucor miehei lipase, Lipozyme RM IM), in solvent-free media at 40 °C. Lipozyme RM IM presented a higher affinity for C10:0 and C10EE. Lipozyme TL IM preferred C10:0 over C8:0 but C8EE over C10EE. Both biocatalysts showed a high activity and operational stability and were not affected by OPO acidity. The New TAG yields ranged 30-60 and the specific productivity ranged 0.96-1.87 g NewTAG/h.g biocatalyst. Lipozyme RM IM cost is more than seven-fold the Lipozyme TL IM cost. Therefore, using Lipozyme TL IM and crude acidic OPO in a continuous bioreactor will contribute to process sustainability for structured lipid production by lowering the cost of the biocatalyst and avoiding oil refining. |
