| Autor: |
Alyamani AA; Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Al-Musawi MH; Department of Clinical Laboratory Science, College of Pharmacy, University of Al-Mustansiriyah, Baghdad 14022, Iraq., Albukhaty S; Department of Chemistry, College of Science, University of Misan, Amarah 62001, Iraq.; College of Medicine, University of Warith Al-Anbiyaa, Karbala 56001, Iraq., Sulaiman GM; Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq., Ibrahim KM; Department of Plant Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad 64074, Iraq., Ahmed EM; Department of Medical Laboratories, College of Applied Medical Sciences, University of Bisha, P.O. Box 551, Bisha 61922, Saudi Arabia.; Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of El Imam El Mahdi, P.O. Box 27711, Kosti 209, Sudan., Jabir MS; Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq., Al-Karagoly H; Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah 58002, Iraq., Aljahmany AA; Department of Medical Basic Sciences, College of Applied Medical Sciences, University of Bisha, P.O. Box 551, Bisha 61922, Saudi Arabia., Mohammed MKA; Radiological Techniques Department, Al-Mustaqbal University College, Hillah 51001, Iraq. |
| Abstrakt: |
The goal of the current work was to create an antibacterial agent by using polycaprolactone/chitosan (PCL/CH) nanofibers loaded with Cordia myxa fruit extract (CMFE) as an antimicrobial agent for wound dressing. Several characteristics, including morphological, physicomechanical, and mechanical characteristics, surface wettability, antibacterial activity, cell viability, and in vitro drug release, were investigated. The inclusion of CMFE in PCL/CH led to increased swelling capability and maximum weight loss. The SEM images of the PCL/CH/CMFE mat showed a uniform topology free of beads and an average fiber diameter of 195.378 nm. Excellent antimicrobial activity was shown towards Escherichia coli (31.34 ± 0.42 mm), Salmonella enterica (30.27 ± 0.57 mm), Staphylococcus aureus (21.31 ± 0.17 mm), Bacillus subtilis (27.53 ± 1.53 mm), and Pseudomonas aeruginosa (22.17 ± 0.12 mm) based on the inhibition zone assay. The sample containing 5 wt% CMFE had a lower water contact angle (47 ± 3.7°), high porosity, and high swelling compared to the neat mat. The release of the 5% CMFE-loaded mat was proven to be based on anomalous non-Fickian diffusion using the Korsmeyer-Peppas model. Compared to the pure PCL membrane, the PCL-CH/CMFE membrane exhibited suitable cytocompatibility on L929 cells. In conclusion, the fabricated antimicrobial nanofibrous films demonstrated high bioavailability, with suitable properties that can be used in wound dressings. |
