SARS-CoV-2 Spike Protein and Neutralizing Anti-Spike Protein Antibodies Modulate Blood Platelet Function.

Autor: Luzak B; Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, 92-215 Lodz, Poland., Rozalski M; Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, 92-215 Lodz, Poland., Przygodzki T; Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, 92-215 Lodz, Poland., Boncler M; Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, 92-215 Lodz, Poland., Wojkowska D; Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, 92-215 Lodz, Poland., Kosmalski M; Department of Clinical Pharmacology, 1st Chair of Internal Diseases, Medical University of Lodz, 90-153 Lodz, Poland., Watala C; Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, 92-215 Lodz, Poland.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Mar 10; Vol. 24 (6). Date of Electronic Publication: 2023 Mar 10.
DOI: 10.3390/ijms24065312
Abstrakt: Several studies report elevated blood platelet activation and altered platelet count in COVID-19 patients, but the role of the SARS-CoV-2 spike protein in this process remains intriguing. Additionally, there is no data that anti-SARS-CoV-2 neutralizing antibodies (nAb) may attenuate spike protein activity toward blood platelets. Our results indicate that under in vitro conditions, the spike protein increased the collagen-stimulated aggregation of isolated platelets and induced the binding of vWF to platelets in ristocetin-treated blood. The spike protein also significantly reduced collagen- or ADP-induced aggregation or decreased GPIIbIIIa (fibrinogen receptor) activation in whole blood, depending on the presence of the anti-spike protein nAb. Our findings suggest that studies on platelet activation/reactivity in COVID-19 patients or in donors vaccinated with anti-SARS-CoV-2 and/or previously-infected COVID-19 should be supported by measurements of spike protein and IgG anti-spike protein antibody concentrations in blood.
Databáze: MEDLINE
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