| Autor: |
Valverde TM; Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil., Soares BNGS; Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil., Nascimento AMD; Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil., Andrade ÂL; Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil., Sousa LRD; Laboratory of Morphopathology, Center for Research in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil., Vieira PMA; Laboratory of Morphopathology, Center for Research in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil., Santos VR; Laboratory of Microbiology and Biomaterials, School of Dentistry, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil., Seibert JB; Natural Products Laboratory, Department of Chemistry, Federal University of São Carlos (UFSCAR), São Carlos 13565-905, SP, Brazil., Almeida TCS; Nuclear Technology Development Center, Belo Horizonte 6627, MG, Brazil., Rodrigues CF; Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil., Oliveira SRM; Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil., Martins FDS; Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, Brazil., Júnior JGF; Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil., Santos VMRD; Department of Chemistry, Federal University of Ouro Preto (UFOP), Ouro Preto 35400-000, MG, Brazil. |
| Abstrakt: |
Many activities have been described for propolis, including, antiviral, antibacterial, antifungal, anti-inflammatory, immunoregulatory, antioxidant and wound healing properties. Recently, propolis has been highlighted due to its potential application in the pharmaceutical and cosmetic industries, motivating a better understanding of its antioxidant and anti-inflammatory activities. Propolis and its main polyphenolic compounds presented high antioxidant activity, and effectiveness as broad spectrum UVB and UVA photoprotection sunscreens. Through a qualitative phytochemical screening, the ethanolic red propolis extracts (EEPV) (70% at room temperature and 70% at a hot temperature) presented a positive result for flavonoids and terpenoids. It presented an antioxidant activity for reducing 50% of DPPH of 17 and 12 μg/mL for extraction at room temperature and at a hot temperature, respectively. The UPLC-QTOF-MS/MS analysis allowed the annotation of 40 substances for EEPV-Heated and 42 substances for EEPV-Room Temperature. The IC 50 results of the ABTS scavenging activity was 4.7 μg/mL for both extractions, at room temperature and at a hot temperature. Additionally, we also evaluated the cytotoxic profile of propolis extracts against macrophage (RAW 264.7 cells) and keratinocytes (HaCaT cells), which showed non-cytotoxic doses in cell viability assays even after a long period of exposure. In addition, propolis extracts showed antibacterial activity for Gram-positive bacteria ( Staphylococcus aureus and Staphylococcus epidermidis ), demonstrating potential biological activity for the creation of formulations aimed at disease control and prevention. |
