| Autor: |
Alonso DP; Biotechnology Institute and Bioscience Institute, Sao Paulo State University, Botucatu 18618-689, Brazil.; Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo 01246-904, Brazil., Amorim JA; Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo 01246-904, Brazil., de Oliveira TMP; Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo 01246-904, Brazil., de Sá ILR; Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo 01246-904, Brazil., Possebon FS; Biotechnology Institute and Bioscience Institute, Sao Paulo State University, Botucatu 18618-689, Brazil., de Carvalho DP; Santo Antônio Energia, Porto Velho 76805-812, Brazil., Ribeiro KAN; Santo Antônio Energia, Porto Velho 76805-812, Brazil., Ribolla PEM; Biotechnology Institute and Bioscience Institute, Sao Paulo State University, Botucatu 18618-689, Brazil., Sallum MAM; Departamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo 01246-904, Brazil. |
| Abstrakt: |
Mosquito females of the genus Mansonia (Blanchard) can be a nuisance to humans and animals since they are voraciously hematophagous and feed on the blood of a variety of vertebrates. Despite their relevance, there is a lack of investigation into the blood-feeding patterns of the Mansonia species. Knowledge of the host preference is crucial in establishing the public health importance of a mosquito species and its potential to be involved in the transmission dynamics of pathogens. Species that are primarily anthropophilic can be more effective in spreading vector-borne pathogens to humans. In this study, we used an Illumina Nextera sequencing protocol and the QIIME2 workflow to assess the diversity of DNA sequences extracted in the ingested blood of mosquito species to evaluate the overall and local host choices for three species: Ma. titillans , Ma. Amazonensis, and Ma. humeralis , in rural areas alongside the Madeira River in the vicinities of the Santo Antonio Energia (SAE) reservoir in the municipality of Porto Velho, Rondônia, Western Brazil. By performing our analysis pipeline, we have found that host diversity per collection site showed a significant heterogeneity across the sample sites. In addition, in rural areas, Ma. amazonensis present a high affinity for B. taurus , Ma. humeralis shows an overall preference for C. familiaris and B. taurus , but also H. sapiens and E. caballus in urban areas, and Ma. titillans showed more opportunistic behavior in rural areas, feeding on wild animals and G. gallus , though with an overall preference for H. sapiens . |
