| Autor: |
Sahu S; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Gupta P; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Gowtham TP; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Yogesh KS; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Sanjay TD; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Singh A; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Duong HV; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India.; Institute of Agricultural Sciences for Southern Vietnam, Ho Chi Minh City 71007, Vietnam., Pradhan SK; ICAR-National Rice Research Institute, Cuttack 753006, India.; Indian Council of Agriculture Research, Krishi Bhawan, New Delhi 110001, India., Bisht DS; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Singh NK; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Baig MJ; ICAR-National Rice Research Institute, Cuttack 753006, India., Rai R; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India., Dash PK; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110001, India. |
| Abstrakt: |
Rice is the major staple food crop for more than 50% of the world's total population, and its production is of immense importance for global food security. As a photophilic plant, its yield is governed by the quality and duration of light. Like all photosynthesizing plants, rice perceives the changes in the intensity of environmental light using phytochromes as photoreceptors, and it initiates a morphological response that is termed as the shade-avoidance response (SAR). Phytochromes (PHYs) are the most important photoreceptor family, and they are primarily responsible for the absorption of the red (R) and far-red (FR) spectra of light. In our endeavor, we identified the morphological differences between two contrasting cultivars of rice: IR-64 (low-light susceptible) and Swarnaprabha (low-light tolerant), and we observed the phenological differences in their growth in response to the reduced light conditions. In order to create genomic resources for low-light tolerant rice, we constructed a subgenomic library of Swarnaprabha that expedited our efforts to isolate light-responsive photoreceptors. The titer of the library was found to be 3.22 × 10 5 cfu/mL, and the constructed library comprised clones of 4-9 kb in length. The library was found to be highly efficient as per the number of recombinant clones. The subgenomic library will serve as a genomic resource for the Gramineae community to isolate photoreceptors and other genes from rice. |
