Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model.

Autor:	Pulido M; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain., de Pedro MÁ; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain.; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain., Álvarez V; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain., Marchena AM; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain.; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain., Blanco-Blázquez V; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain.; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain.; CIBER de Enfermedades Cardiovasculares (CIBERCV), C. de Melchor Fernández Almagro, 3, 28029 Madrid, Spain., Báez-Díaz C; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain.; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain.; CIBER de Enfermedades Cardiovasculares (CIBERCV), C. de Melchor Fernández Almagro, 3, 28029 Madrid, Spain., Crisóstomo V; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain.; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain.; CIBER de Enfermedades Cardiovasculares (CIBERCV), C. de Melchor Fernández Almagro, 3, 28029 Madrid, Spain., Casado JG; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain.; Immunology Unit, University of Extremadura, Campus Universitario, Av. de la Universidad, s/n, 10003 Cáceres, Spain.; Institute of Molecular Pathology Biomarkers, University of Extremadura, 10003 Cáceres, Spain., Sánchez-Margallo FM; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain.; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain.; CIBER de Enfermedades Cardiovasculares (CIBERCV), C. de Melchor Fernández Almagro, 3, 28029 Madrid, Spain., López E; Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, Spain.; RICORS-TERAV Network, ISCIII, 28029 Madrid, Spain.
Jazyk:	angličtina
Zdroj:	Biology [Biology (Basel)] 2023 Feb 21; Vol. 12 (3). Date of Electronic Publication: 2023 Feb 21.
DOI:	10.3390/biology12030340
Abstrakt:	Acute myocardial infarction (AMI) is the consequence of an acute interruption of myocardial blood flow delimiting an area with ischemic necrosis. The loss of cardiomyocytes initiates cardiac remodeling in the myocardium, leading to molecular changes in an attempt to recover myocardial function. The purpose of this study was to unravel the differences in the molecular profile between ischemic and remote myocardium after AMI in an experimental model. To mimic human myocardial infarction, healthy pigs were subjected to occlusion of the mid-left anterior descending coronary artery, and myocardial tissue was collected from ischemic and remote zones for omics techniques. Comparative transcriptome analysis of both areas was accurately validated by proteomic analysis, resulting in mitochondrion-related biological processes being the most impaired mechanisms in the infarcted area. Moreover, Immune system process-related genes were up-regulated in the remote tissue, mainly due to the increase of neutrophil migration in this area. These results provide valuable information regarding differentially expressed genes and their biological functions between ischemic and remote myocardium after AMI, which could be useful for establishing therapeutic targets for the development of new treatments.
Databáze:	MEDLINE
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