| Autor: |
Meshalkina D; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia.; Sechenov Institute of Evolutional Physiology and Biochemistry, 194223 St. Petersburg, Russia., Tsvetkova E; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia.; Department of General Pathology and Pathological Physiology, Institute of Experimental Medicine, 197022 St. Petersburg, Russia., Orlova A; Laboratory of Analytical Biochemistry and Biotechnology, K.A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, Russia., Islamova R; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Grashina M; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Gorbach D; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia., Babakov V; Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medicobiological Agency, 188663 St. Petersburg, Russia., Francioso A; Department of Biochemical Sciences, Sapienza University, 00185 Roma, Italy.; Department of Bioscience and Technology for Food, Agriculture and Environment University of Teramo, 64100 Teramo, Italy., Birkemeyer C; Faculty of Chemistry and Mineralogy, University of Leipzig, 04103 Leipzig, Germany.; German Center for Integrative Biodiversity Research (iDiv), 04103 Leipzig, Germany., Mosca L; Department of Bioscience and Technology for Food, Agriculture and Environment University of Teramo, 64100 Teramo, Italy., Tarakhovskaya E; Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia.; Vavilov Institute of General Genetics RAS, St. Petersburg Branch, 190000 St. Petersburg, Russia., Frolov A; Laboratory of Analytical Biochemistry and Biotechnology, K.A. Timiryazev Institute of Plant Physiology RAS, 127276 Moscow, Russia. |
| Abstrakt: |
Phaeophyceae (brown algae) essentially contribute to biotopes of cold and temperate seas. Their thalli are rich in biologically active natural products, which are strongly and universally dominated with phlorotannins-polyphenols of complex and diverse structure based on multiple differently arranged phloroglucinol units and well known as strong antioxidants with a broad spectrum of biological activities. In the algal cells, phlorotannins can either accumulate in the cytoplasm or can be secreted into the cell wall (CW). The biological activities of extractable intracellular phlorotannins have been comprehensively characterized, whereas the properties of the CW-bound polyphenol fraction are still mostly unknown. Recently, we identified dibenzodioxin bonding as the principal structural feature of the CW-bound phlorotannins in fucoid algae, whereas soluble intracellular phlorotannins rely on aryl and ether bonds. However, profiles of biological activity associated with these structural differences are still unknown. Therefore, to the best of our knowledge, for the first time we address the antioxidant, cytotoxic, neuroprotective, and antibacterial properties of the CW-bound phlorotannin fractions isolated from two representatives of the order Fucales- Fucus vesiculosus and Pelvetia canaliculata . The CW-bound phlorotannins appeared to be softer antioxidants, stronger antibacterial agents and were featured with essentially less cytotoxicity in comparison to the intracellular fraction. However, the neuroprotective effects of both sub-cellular phlorotannin fractions of F. vesiculosus and P. canaliculata were similar. Thus, due to their lower cytotoxicity, CW-bound phlorotannins can be considered as promising antioxidants and neuroprotectors. |
