Topiramate affords neuroprotection in diabetic neuropathy model via downregulating spinal GFAP/inflammatory burden and improving neurofilament production.

Autor: Attia MA; Department of Pharmacology, College of Medicine, AlMaarefa University, Riyadh, Saudi Arabia.; Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Soliman N; Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.; Center of Excellence of Molecular and Cellular Medicine, Suez Canal University, Ismailia, Egypt., Eladl MA; Department of Basic Medical Sciences, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates., Bilasy SE; Department of Biochemistry, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.; College of Dental Medicine, California Northstate University, Elk Grove, CA, USA., El-Abaseri TB; Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Ali HS; Department of Pharmacology, Faculty of Medicine, Assiut University, Assiut, Egypt.; Department of Pharmacology, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia., Abbas F; Physiology department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Ibrahim D; Physiology department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Osman NMS; Department of Human Anatomy and Embryology, Faculty of Medicine, Port Said University, Port Said, Egypt., Hashish AA; Basic Medical Sciences Department, College of Medicine, University of Bisha, Bisha, Saudi Arabia.; Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Alshahrani A; Department of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha, KSA., Mohamed AS; Faculty of Public Health and Tropical Medicine, Jazan University, Jazan, Saudi Arabia., Zaitone SA; Deparment of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi Arabia.; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
Jazyk: angličtina
Zdroj: Toxicology mechanisms and methods [Toxicol Mech Methods] 2023 Sep; Vol. 33 (7), pp. 563-577. Date of Electronic Publication: 2023 Apr 20.
DOI: 10.1080/15376516.2023.2196687
Abstrakt: The current study aimed to test the neuroprotective action of topiramate in mouse peripheral diabetic neuropathy (DN) and explored some mechanisms underlying this action. Mice were assigned as vehicle group, DN group, DN + topiramate 10-mg/kg and DN + topiramate 30-mg/kg. Mice were tested for allodynia and hyperalgesia and then spinal cord and sciatic nerves specimens were examined microscopically and neurofilament heavy chain (NEFH) immunostaining was performed. Results indicated that DN mice had lower the hotplate latency time (0.46-fold of latency to licking) and lower von-Frey test pain threshold (0.6-fold of filament size) while treatment with topiramate increased these values significantly. Sciatic nerves from DN control mice showed axonal degeneration while spinal cords showed elevated GFAP (5.6-fold) and inflammatory cytokines (∼3- to 4-fold) but lower plasticity as indicated by GAP-43 (0.25-fold). Topiramate produced neuroprotection and suppressed spinal cord GFAP/inflammation but enhanced GAP-43. This study reinforces topiramate as neuroprotection and explained some mechanisms included in alleviating neuropathy.
Databáze: MEDLINE
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