A RP-HPLC-DAD method for analysis of Brazilian southeast brown propolis and its leishmanicidal properties.

Autor: Ribeiro VP; Research Group on Natural Products, Center for Research in Sciences and Technology, University of Franca, Franca, SP, Brazil., Aldana-Mejia JA; School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo, Ribeirão Preto, Brazil., Arruda C; School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo, Ribeirão Preto, Brazil., Candido ACBB; Research Group on Natural Products, Center for Research in Sciences and Technology, University of Franca, Franca, SP, Brazil., Magalhães LG; Research Group on Natural Products, Center for Research in Sciences and Technology, University of Franca, Franca, SP, Brazil.; Animal Science Post Graduation, University of Franca, Franca, SP, Brazil., Oliveira ND; Animal Science Post Graduation, University of Franca, Franca, SP, Brazil., Veneziani RCS; Research Group on Natural Products, Center for Research in Sciences and Technology, University of Franca, Franca, SP, Brazil., Bastos JK; School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo, Ribeirão Preto, Brazil., Ambrósio SR; Research Group on Natural Products, Center for Research in Sciences and Technology, University of Franca, Franca, SP, Brazil.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2023 Aug; Vol. 37 (8), pp. e5634. Date of Electronic Publication: 2023 Apr 18.
DOI: 10.1002/bmc.5634
Abstrakt: Propolis is a natural product of great economic and pharmacological importance. The flora surrounding the bee communities is a determining factor in the composition of propolis and therefore in its biological and medicinal properties. Brown propolis is one of the most important types of propolis in Brazil, produced in the southeastern region. The ethanolic extract of a brown propolis sample from Minas Gerais state was chemically characterized for the subsequent development of a RP-HPLC method, validated according to the standards of regulatory agencies. The leishmanicidal activity of this extract was assessed. The brown propolis was characterized by the presence of chemical markers reported on green propolis such as ferulic acid, coumaric acid, caffeic acid, cinnamic acid, baccharin, artepillin and drupanin, indicating a probable origin on Baccharis dracunculifolia. The developed method agreed with the parameters established by the validation guidelines, then proved to be reliable for the analysis of this type of propolis. The brown propolis displayed significant activity against Leishmania amazonensis with IC 50 values of 1.8 and 2.4 μg/ml against the promastigote and amastigote forms, respectively. The studied propolis exhibited promising evidence for use as a natural source against L. amazonensis.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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