| Autor: |
Park H; Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.; Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea., Cho Y; Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.; Department of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, Republic of Korea., Lee J; KnA Consulting, Yongin-si 16942, Republic of Korea., Lee KM; Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea., Kim HJ; Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea., Lee J; Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea., Bahn YS; Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea., Son J; Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea. |
| Abstrakt: |
Ptaquiloside, a naturally occurring cancer-causing substance in bracken fern, has been detected in the meat and milk of cows fed a diet containing bracken fern. A rapid and sensitive method for the quantitative analysis of ptaquiloside in bracken fern, meat, and dairy products was developed using the QuEChERS method and liquid chromatography-tandem mass spectrometry. The method was validated according to the Association of Official Analytical Chemists guidelines and met the criteria. A single matrix-matched calibration method with bracken fern has been proposed, which is a novel strategy that uses one calibration for multiple matrices. The calibration curve ranged from 0.1 to 50 µg/kg and showed good linearity ( r 2 > 0.99). The limits of detection and quantification were 0.03 and 0.09 µg/kg, respectively. The intraday and interday accuracies were 83.5-98.5%, and the precision was <9.0%. This method was used for the monitoring and exposure assessment of ptaquiloside in all routes of exposure. A total of 0.1 µg/kg of ptaquiloside was detected in free-range beef, and the daily dietary exposure of South Koreans to ptaquiloside was estimated at up to 3.0 × 10 -5 µg/kg b.w./day. The significance of this study is to evaluate commercially available products in which ptaquiloside may be present, to monitor consumer safety. |
