| Autor: |
Halfter N; Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, Germany., Espinosa-Cano E; Group of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, C/Monforte de Lemos 3/5, 28029 Madrid, Spain., Pontes-Quero GM; Group of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, C/Monforte de Lemos 3/5, 28029 Madrid, Spain., Ramírez-Jiménez RA; Group of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, C/Monforte de Lemos 3/5, 28029 Madrid, Spain., Heinemann C; Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, Germany., Möller S; Department of Biomaterials, INNOVENT e. V., Prüssingstraße 27B, 07745 Jena, Germany., Schnabelrauch M; Department of Biomaterials, INNOVENT e. V., Prüssingstraße 27B, 07745 Jena, Germany., Wiesmann HP; Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, Germany., Hintze V; Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, Germany., Aguilar MR; Group of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, Spain.; CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, C/Monforte de Lemos 3/5, 28029 Madrid, Spain. |
| Abstrakt: |
Current limitations of wound dressings for treating chronic wounds require the development of novel approaches. One of these is the immune-centered approach, which aims to restore the pro-regenerative and anti-inflammatory properties of macrophages. Under inflammatory conditions, ketoprofen nanoparticles (KT NPs) can reduce pro-inflammatory markers of macrophages and increase anti-inflammatory cytokines. To assess their suitability as part of wound dressings, these NPs were combined with hyaluronan (HA)/collagen-based hydro- (HGs) and cryogels (CGs). Different HA and NP concentrations and loading techniques for NP incorporation were used. The NP release, gel morphology, and mechanical properties were studied. Generally, colonialization of the gels with macrophages resulted in high cell viability and proliferation. Furthermore, direct contact of the NPs to the cells reduced the level of nitric oxide (NO). The formation of multinucleated cells on the gels was low and further decreased by the NPs. For the HGs that produced the highest reduction in NO, extended ELISA studies showed reduced levels of the pro-inflammatory markers PGE2, IL-12 p40, TNF-α, and IL-6. Thus, HA/collagen-based gels containing KT NPs may represent a novel therapeutic approach for treating chronic wounds. Whether effects observed in vitro translate into a favorable profile on skin regeneration in vivo will require rigorous testing. |
