| Autor: |
Fracasso JAR; School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil., Ibe MB; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil., da Costa LTS; School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil.; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil.; Department of Biotechnology and Bioprocesses Engineering, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil., Guarnier LP; Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14048-900, Brazil., Viel AM; Nossa Senhora do Patrocínio Faculty Center-CEUNSP, Itu 13300-200, Brazil., Brito GR; Department of Biology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil., Parron MC; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil., Pereira AEDS; Institute of Science and Technology of Sorocaba, São Paulo State University (UNESP), Sorocaba 18087-180, Brazil., Pegorin Brasil GS; Department of Biotechnology and Bioprocesses Engineering, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil.; Department of Biochemistry and Chemical Technology, Institute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-060, Brazil., Farias Ximenes V; School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil.; Department of Chemistry, Faculty of Sciences, São Paulo State University (UNESP), Bauru 17033-360, Brazil., Fraceto LF; Institute of Science and Technology of Sorocaba, São Paulo State University (UNESP), Sorocaba 18087-180, Brazil., Malacrida Mayer CR; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil., Ribeiro-Paes JT; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil.; Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14048-900, Brazil., Ferreira FY; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil., Zoppe NA; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil., Santos LD; School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil.; Department of Biotechnology, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil.; Department of Biotechnology and Bioprocesses Engineering, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Araraquara 14800-903, Brazil. |
| Abstrakt: |
Caryocar brasiliense Cambess is a plant species typical of the Cerrado, a Brazilian biome. The fruit of this species is popularly known as pequi, and its oil is used in traditional medicine. However, an important factor hindering the use of pequi oil is its low yield when extracted from the pulp of this fruit. Therefore, in this study, with aim of developing a new herbal medicine, we an-alyzed the toxicity and anti-inflammatory activity of an extract of pequi pulp residue (EPPR), fol-lowing the mechanical extraction of the oil from its pulp. For this purpose, EPPR was prepared and encapsulated in chitosan. The nanoparticles were analyzed, and the cytotoxicity of the encapsu-lated EPPR was evaluated in vitro. After confirming the cytotoxicity of the encapsulated EPPR, the following evaluations were performed with non-encapsulated EPPR: in vitro anti-inflammatory activity, quantification of cytokines, and acute toxicity in vivo. Once the anti-inflammatory activity and absence of toxicity of EPPR were verified, a gel formulation of EPPR was developed for topical use and analyzed for its in vivo anti-inflammatory potential, ocular toxicity, and previous stability assessment. EPPR and the gel containing EPPR showed effective anti-inflammatory activity and lack of toxicity. The formulation was stable. Thus, a new herbal medicine with anti-inflammatory activity can be developed from discarded pequi residue. |
