Establishment of Epithelial Cell Culture from Ovarian Cancer Tissues: A Method Comparison Study.

Autor:	Anggraeni TD; Doctoral Program of Medical Science, Faculty of Medicine Universitas Indonesia, DKI Jakarta, Indonesia.; Gynecological Oncology Division, Department of Obstetric Gynecologic, Faculty of Medicine Universitas Indonesia, DKI Jakarta, Indonesia., Hariadi A; ORCA Bioteknologi Nusantara, DKI Jakarta, Indonesia.; Human Cancer Research Center, Institute of Medical Research, Universitas Indonesia, DKI Jakarta, Indonesia., Kispa TD; ORCA Bioteknologi Nusantara, DKI Jakarta, Indonesia., Thiono J; Gynecological Oncology Division, Department of Obstetric Gynecologic, Faculty of Medicine Universitas Indonesia, DKI Jakarta, Indonesia., Andrijono A; Gynecological Oncology Division, Department of Obstetric Gynecologic, Faculty of Medicine Universitas Indonesia, DKI Jakarta, Indonesia., Winarto H; Gynecological Oncology Division, Department of Obstetric Gynecologic, Faculty of Medicine Universitas Indonesia, DKI Jakarta, Indonesia., Tan MI; School of Life Science and Technology, Bandung Institute of Technology, Bandung, West Java, Indonesia.
Jazyk:	angličtina
Zdroj:	Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2023 Mar 01; Vol. 24 (3), pp. 1047-1054. Date of Electronic Publication: 2023 Mar 01.
DOI:	10.31557/APJCP.2023.24.3.1047
Abstrakt:	Objective: This study aimed to determine the most effective method in establishing primary cell culture from epithelial serous ovarian cancer tissues with the highest yield of cells and percentage of epithelial cells. Methods: Primary and metastasis tissues from three serous ovarian cancer patients were processed using 18 different combinations of methods based on different factors: the source of tissue (primary site or metastasis site), the cell suspension method (explant method, enzymatic methods, or the addition of Percoll), and the alternatives of three different media. We compared the total count of cells, the percentage of epithelial cells, and the estimated number of epithelial cells per observation field. The calculation of cells from primary tissues were compared to metastasis tissues, and the difference was statistically analyzed using Mann Whitney-U test on SPSS software. Result: The groups that were processed using dispase and trypsin resulted higher number of cells and higher percentage of epithelial cells when compared to the explant method. Among all media, we found that DMEM:F12 and McCoy's 5A media as equally useful in isolating and culturing epithelial cells. Statistically, the metastasis tissue derived more epithelial cells when compared to the primary tissue (102.32±82.65 vs 22.6±23.81, p=0.001). Conclusion: The use of metastasis tissue processed with trypsin or dispase and cultured in DMEM:F12 or McCoy's 5A media was found to be the most efficient way to produce the highest amount of cells with high percentage of epithelial cells.
Databáze:	MEDLINE
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