CRISPR-assisted test for Schistosoma haematobium.

Autor: Cherkaoui D; London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK. dounia.cherkaoui.17@ucl.ac.uk.; Division of Medicine, University College London, London, WC1E 6BT, UK. dounia.cherkaoui.17@ucl.ac.uk., Mesquita SG; Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK.; René Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte, Minas Gerais, Brazil.; London Centre for Neglected Tropical Disease Research (LCNTDR), London, W21 PG, UK., Huang D; London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK., Lugli EB; Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK.; London Centre for Neglected Tropical Disease Research (LCNTDR), London, W21 PG, UK., Webster BL; Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK. b.webster@nhm.ac.uk.; London Centre for Neglected Tropical Disease Research (LCNTDR), London, W21 PG, UK. b.webster@nhm.ac.uk., McKendry RA; London Centre for Nanotechnology, University College London, London, WC1H 0AH, UK. r.a.mckendry@ucl.ac.uk.; Division of Medicine, University College London, London, WC1E 6BT, UK. r.a.mckendry@ucl.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Mar 27; Vol. 13 (1), pp. 4990. Date of Electronic Publication: 2023 Mar 27.
DOI: 10.1038/s41598-023-31238-y
Abstrakt: Schistosomiasis is a major neglected tropical disease targeted for elimination as a public health issue by 2030, however there is an urgent need for more sensitive and specific diagnostic tests suitable to resource-limited settings. Here we developed CATSH, a CRISPR-assisted diagnostic test for Schistosoma haematobium, utilising recombinase polymerase amplification, Cas12a-targeted cleavage and portable real-time fluorescence detection. CATSH showed high analytical sensitivity, consistent detection of a single parasitic egg and specificity for urogenital Schistosoma species. Thanks to a novel CRISPR-compatible sample preparation developed using simulated urine samples containing parasitic eggs, CATSH had a sample-to-result within 2 h. The components of CATSH can be lyophilised, reducing cold chain dependence and widening access to lower and middle-income countries. This work presents a new application of CRISPR diagnostics for highly sensitive and specific detection of parasitic pathogens in remote areas and could have a significant impact on the elimination of neglected tropical diseases.
(© 2023. The Author(s).)
Databáze: MEDLINE
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