Acute and sub-acute toxicity evaluation of Merremia tridentata (L.) stem extract on mice.

Autor: Canh Pham E; Department of Medicinal Chemistry, Faculty of Pharmacy, Hong Bang International University, 700000, Ho Chi Minh City, Viet Nam. Electronic address: canhem112009@gmail.com., Van LV; Department of Pharmaceutics - Pharmaceutical Industry, Faculty of Pharmacy, Lac Hong University, 810000, Dong Nai Province, Viet Nam., Nguyen CV; Department of Analytical Chemistry and Drug Quality Control, Faculty of Pharmacy, Lac Hong University, 810000, Dong Nai Province, Viet Nam., Duong NTN; Department of Pharmacology - Clinical Pharmacy, Faculty of Pharmacy, Lac Hong University, 810000, Dong Nai Province, Viet Nam., Le Thi TV; Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, 700000, Ho Chi Minh City, Viet Nam. Electronic address: lttvi112113@gmail.com., Truong TN; Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, 700000, Ho Chi Minh City, Viet Nam. Electronic address: truongtuyen@ump.edu.vn.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2023 May; Vol. 227, pp. 107093. Date of Electronic Publication: 2023 Mar 25.
DOI: 10.1016/j.toxicon.2023.107093
Abstrakt: The acute and sub-acute toxicity studies were performed in male and female Swiss albino mice as per the guidelines mentioned in OECD. The oral administration of M. tridentata stem extract (MSE) showed no treatment-related mortality and body weight change in mice up to the single dose of 30,000 mg/kg body weight in the acute toxicity study and up to a dose of 30,000 mg/kg/day body weight in the sub-acute toxicity study. Moreover, the clinical signs, body weight, gross pathology, organ weight, hematology (except for platelet count), biochemical analysis, and histopathology did not show significant variation at a medium dose of 15,000 mg/kg/day body weight compared to the control group. However, toxicological signs in behavior, very mild interstitial nephritis, as well as significant variation in platelet count and total protein parameters were observed at a dose of 30,000 mg/kg/day in the 28-day oral toxicity study. Thus, the no-observed-adverse-effect level was determined at a dose of 15,000 mg/kg/day body weight. Based on study results, it is concluded that MSE showed LD 50 of greater than 5000 mg/kg/day body weight. Hence, this could be a potential candidature as a future safe pharmaceutical product.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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