Determination of Rutin's antitumoral effect on EAC solid tumor by AgNOR count and PI3K/AKT/mTOR signaling pathway.

Autor: Yılmaz S; Department of Anatomy, Faculty of Medicine, Yozgat Bozok University, 66100, Yozgat, Turkey. sehery38@hotmail.com., Doğanyiğit Z; Department of Histology and Embriology, Faculty of Medicine, Yozgat Bozok University, 66100, Yozgat, Turkey., Oflamaz AO; Department of Histology and Embriology, Faculty of Medicine, Yozgat Bozok University, 66100, Yozgat, Turkey., Ateş Ş; Department of Anatomy, Faculty of Medicine, Yozgat Bozok University, 66100, Yozgat, Turkey., Söylemez ESA; Department of Medical Biology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03100, Afyon, Turkey., Nisari M; Department of Anatomy, Faculty of Medicine, Erciyes University, Kayseri, Turkey., Farooqı AA; Institute of Biomedical and Genetic Engineering (IBGE), Islamabad, Pakistan.
Jazyk: angličtina
Zdroj: Medical oncology (Northwood, London, England) [Med Oncol] 2023 Mar 27; Vol. 40 (5), pp. 131. Date of Electronic Publication: 2023 Mar 27.
DOI: 10.1007/s12032-023-01999-7
Abstrakt: Rutin is one of the flavonoids found in fruits and vegetables. The PI3K/AKT/mTOR signaling pathway is critical for the life cycle at the cellular level. In current study, we purposed to demonstrate the antitumoral effect of rutin at different doses through the mTOR-signaling pathway and argyrophilic nucleolar regulatory region. EAC cells were injected subcutaneously into the experimental groups. 25 and 50 mg/kg Rutin were injected intraperitoneally to the animals with solid tumors for 14 days. Immunohistochemical, Real-time PCR and AgNOR analyzes were actualized on the taken tumors. When the rutin given groups and the tumor group were compared, the tumor size increase was detected to be statistically significant (p < 0.05). In immunohistochemical analysis, a significant decrease was encountered in the AKT, mTOR, PI3K and F8 expressions especially in the groups administered 25 mg Rutin, in comparison with the control group (p < 0.05). AgNOR area/nuclear area (TAA/NA) and average AgNOR number were determineted, and statistically important differences were detected between the groups in terms of TAA/NA ratio (p < 0.05). There were significant statistical differences between the mRNA quantity of the PI3K, AKT1 and mTOR genes (p < 0.05). In the in vitro study, cell apoptosis was evaluated with different doses of annexin V and it was determined that a dose of 10 µg/mL Rutin induced apoptosis (p < 0.05). In our study, it was demonstrated in vivo and in vitro that Rutin has an anti-tumor effect on the development of solid tumors formed by both EAC cells.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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