| Autor: |
Mantri M; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York., Hinchman MM; Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York., McKellar DW; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York., Wang MFZ; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York., Cross ST; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York.; Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York.; Cornell Institute for Host-Microbe Interactions and Disease, Cornell University, Ithaca, New York., Parker JSL; Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York.; Cornell Institute for Host-Microbe Interactions and Disease, Cornell University, Ithaca, New York., De Vlaminck I; Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York.; Cornell Institute for Host-Microbe Interactions and Disease, Cornell University, Ithaca, New York. |
| Abstrakt: |
A significant fraction of sudden death in children and young adults is due to viral myocarditis, an inflammatory disease of the heart. In this study, by using integrated single-cell and spatial transcriptomics, we created a high-resolution, spatially resolved transcriptome map of reovirus-induced myocarditis in neonatal mouse hearts. We assayed hearts collected at three timepoints after infection and studied the temporal, spatial and cellular heterogeneity of host-virus interactions. We further assayed the intestine, the primary site of reovirus infection, to establish a full chronology of molecular events that ultimately lead to myocarditis. We found that inflamed endothelial cells recruit cytotoxic T cells and undergo pyroptosis in the myocarditic tissue. Analyses of spatially restricted gene expression in myocarditic regions and the border zone identified immune-mediated cell-type-specific injury and stress responses. Overall, we observed a complex network of cellular phenotypes and spatially restricted cell-cell interactions associated with reovirus-induced myocarditis in neonatal mice. |
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