Oxidative stress and toxicity produced by arsenic and chromium in broiler chicks and application of vitamin E and bentonite as ameliorating agents.

Autor:	Mashkoor J; Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan., Al-Saeed FA; Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia., Guangbin Z; Shandong Vocational Animal Science and Veterinary College, Weifang, China., Alsayeqh AF; Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Qassim, Saudi Arabia., Gul ST; Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan., Hussain R; Department of Pathology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan., Ahmad L; Department of Pre-clinical Studies, Faculty of Veterinary Medicine, Baqai Medical University, Karachi, Pakistan., Mustafa R; University of Agriculture, Faisalabad Sub Campus, Toba Tek Singh, Pakistan., Farooq U; University of Agriculture, Faisalabad Sub Campus, Toba Tek Singh, Pakistan., Khan A; Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.; Shandong Vocational Animal Science and Veterinary College, Weifang, China.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 Mar 08; Vol. 10, pp. 1128522. Date of Electronic Publication: 2023 Mar 08 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1128522
Abstrakt:	The present study investigated the adverse effects of arsenic and chromium in broilers and ascertained the role of vitamin E and bentonite in alleviating their harmful effects. For this purpose, we experimented on 180 one-day-old broiler chickens. The feed was administered to broiler chicks of groups 2, 6, 7, 8, and 9 chromium @ (270 mg.kg -1 BW). Groups 3, 6, 7, 8, and 9 were administered arsenic @ (50 mg.kg -1 BW). Groups 4, 7, and 9 received vitamin E (150 mg.kg -1 BW), and groups 5, 8, and 9 received bentonite (5%), respectively. Group 1 was kept in control. All the broiler chicks treated with chromium and arsenic showed a significant ( p < 0.05) decline in erythrocytic parameters on experimental days 21 and 42. Total proteins decreased significantly, while ALT, AST, urea, and creatinine increased significantly ( p < 0.05). TAC and CAT decreased significantly ( p < 0.05), while TOC and MDA concentrations increased significantly ( p < 0.05) in chromium and arsenic-treated groups on experimental days 21 and 42. Pearson correlation analysis revealed a strong positive correlation between TAC and CAT (Pearson correlation value = 0.961; p < 0.001), similarly TOC and MDA positive correlation (Pearson correlation value = 0.920; p < 0.001). However, TAC and CAT showed a negative correlation between TOC and MDA. The intensity of gross and microscopic lesions was more in chromium (270 mg.kg -1 ) and arsenic (50 mg.kg -1 ) singly or in combination-treated groups. Thus, broiler chicks treated with chromium plus arsenic exhibited higher gross and microscopic lesion intensity than other treated groups. Fatty degeneration, severe cytoplasmic vacuolar degeneration, and expansion of sinusoidal spaces were the main lesions observed in the liver. Kidneys showed renal epithelial cells necrosis, glomerular shrinkage, and severe cytoplasmic vacuolar degeneration. Co-administration of bentonite along with chromium and arsenic resulted in partial amelioration (group 8) compared to groups 7 and 9, administered arsenic + chromium + vitamin E and arsenic + chromium + vitamin E + bentonite, respectively. It was concluded that arsenic and chromium cause damage not only to haemato-biochemical parameters but also lead to oxidation stress in broilers. Vitamin E and bentonite administration can ameliorate toxicity and oxidative stress produced by arsenic and chromium. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Mashkoor, Al-Saeed, Guangbin, Alsayeqh, Gul, Hussain, Ahmad, Mustafa, Farooq and Khan.)
Databáze:	MEDLINE
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