Using mass spectrometry imaging to visualize age-related subcellular disruption.

Autor:	Hogan KA; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States.; Signal Transduction and Molecular Nutrition Laboratory, Kogod Aging Center, Department of Anesthesiology and Perioperative Medicine, Mayo Clinic College of Medicine, Rochester, MN, United States.; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, United States., Zeidler JD; Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, United States., Alsaadi AI; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States., Alshaheeb AA; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States., Chang YC; Department of Chemistry, Pennsylvania State University, University Park, PA, United States., Tian H; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, United States.; Department of Chemistry, Pennsylvania State University, University Park, PA, United States., Hinton AO Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, United States., McReynolds MR; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States.; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in molecular biosciences [Front Mol Biosci] 2023 Mar 08; Vol. 10, pp. 906606. Date of Electronic Publication: 2023 Mar 08 (Print Publication: 2023).
DOI:	10.3389/fmolb.2023.906606
Abstrakt:	Metabolic homeostasis balances the production and consumption of energetic molecules to maintain active, healthy cells. Cellular stress, which disrupts metabolism and leads to the loss of cellular homeostasis, is important in age-related diseases. We focus here on the role of organelle dysfunction in age-related diseases, including the roles of energy deficiencies, mitochondrial dysfunction, endoplasmic reticulum (ER) stress, changes in metabolic flux in aging (e.g., Ca 2+ and nicotinamide adenine dinucleotide), and alterations in the endoplasmic reticulum-mitochondria contact sites that regulate the trafficking of metabolites. Tools for single-cell resolution of metabolite pools and metabolic flux in animal models of aging and age-related diseases are urgently needed. High-resolution mass spectrometry imaging (MSI) provides a revolutionary approach for capturing the metabolic states of individual cells and cellular interactions without the dissociation of tissues. mass spectrometry imaging can be a powerful tool to elucidate the role of stress-induced cellular dysfunction in aging. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Hogan, Zeidler, Beasley, Alsaadi, Alshaheeb, Chang, Tian, Hinton and McReynolds.)
Databáze:	MEDLINE
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