Formulation and evaluation of alginate-gelatin hydrogel scaffolds loaded with zinc-doped hydroxyapatite and 5-fluorouracil.

Autor: Elsayed EW; Medicinal and Pharmaceutical Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, P.O.12622, 33 EL Bohouth St. (former EL Tahrir St.), Dokki, Giza, Egypt. Electronic address: ebtesam.wahman@yahoo.com., El-Ashmawy AA; Medicinal and Pharmaceutical Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, P.O.12622, 33 EL Bohouth St. (former EL Tahrir St.), Dokki, Giza, Egypt., El-Bassyouni GT; Refractories, Ceramics and Building Materials Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research Centre, P.O.12622, 33 EL Bohouth St. (former EL Tahrir St.), Dokki, Giza, Egypt., Mousa SM; Inorganic Chemistry Department, Advanced Materials Technology and Mineral Resources Research Institute, National Research Centre, P.O.12622, 33 EL Bohouth St. (former EL Tahrir St.), Dokki, Giza, Egypt., El-Manawaty M; Pharmacognosy Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, P.O.12622, 33 EL Bohouth St. (former EL Tahrir St.), Dokki, Giza, Egypt., Emara LH; Medicinal and Pharmaceutical Chemistry Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, P.O.12622, 33 EL Bohouth St. (former EL Tahrir St.), Dokki, Giza, Egypt.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 May 15; Vol. 237, pp. 124147. Date of Electronic Publication: 2023 Mar 23.
DOI: 10.1016/j.ijbiomac.2023.124147
Abstrakt: Alginate and gelatin are natural macromolecules used to formulate biocompatible drug delivery systems. Hydroxyapatite (HA) is an osteophilic ceramic used to prepare bone scaffolds. The current study aimed at preparing and characterizing HA, zinc-doped HA, and 5-fluorouracil(5-FU)-loaded alginate-gelatin-based hydrogel scaffolds using different crosslinking solutions. 5-FU incorporation efficiency, in-vitro drug release, antitumor bioassays, FTIR, X-ray-diffraction (XRD), High-Resolution Transmission, and Scanning-Electron Microscope (HR-TEM and SEM) studies were conducted. XRD showed the incorporation of Zn 2+ into HA structure with a deformity in HA crystal lattice and inhibited crystal growth. FTIR-spectra represented the characteristic bands corresponding to HA structure. HR-TEM showed a decreased HA crystal size and rod-like crystallites that increased with increasing zinc content. Zn 2+ content and 5-FU-loading caused significant effects on the scaffolds' thickness (p-value = 0.021 and 0.035, respectively). Burst 5-FU release within 10-15 min followed by 100 % release within 4 h was observed. Zinc content showed a significant positive effect on the cytotoxicity% of the blank and drug-loaded scaffolds. XRD and FTIR studies revealed that 5-FU was completely incorporated into the hydrogel with no chemical interaction. SEM-imaging showed interconnected pores and needle-shaped drug particles. The prepared formulations showed promising physico-chemical properties for targeted delivery of 5-FU in the form of biocompatible bone scaffolds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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