Determination of creatinine in urine and blood serum human samples by CZE-UV using on-column internal standard injection.

Autor: de Oliveira Moreira OB; Grupo de Química Analítica e Quimiometria - GQAQ, Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brazil., Queiroz de Souza JC; Grupo de Química Analítica e Quimiometria - GQAQ, Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brazil., Beraldo Candido JM; Grupo de Química Analítica e Quimiometria - GQAQ, Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brazil., do Nascimento MP; Grupo de Química Analítica e Quimiometria - GQAQ, Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brazil., Chellini PR; Grupo de Química Analítica e Quimiometria - GQAQ, Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brazil; Faculdade de Farmácia, Federal University of Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brazil., de Lemos LM; Lemos Laboratório de Análises Clínicas, 36025-290, Juiz de Fora, MG, Brazil., de Oliveira MAL; Grupo de Química Analítica e Quimiometria - GQAQ, Chemistry Department, Institute of Exact Sciences, Federal University of Juiz de Fora (UFJF), 36036-900, Juiz de Fora, MG, Brazil; National Institute of Science and Technology for Bioanalytics - INCTBio, Institute of Chemistry, University of Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil. Electronic address: marcone.oliveira@ufjf.br.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2023 Jun 01; Vol. 258, pp. 124465. Date of Electronic Publication: 2023 Mar 21.
DOI: 10.1016/j.talanta.2023.124465
Abstrakt: Creatinine is a well-stablished biomarker for kidney malfunctions and for normalization parameter of urinary quantitative information. Recently, metabolic studies have been discovering other functionalities for creatinine tests in human urine and blood serum. In this work we present an enhanced capillary electrophoresis (CE) based protocol for determination of creatinine. CE is a high-throughput separation technique that have been getting attention through the last decades and might be considered to be adopted as an analytical instrumentation for clinical purposes. In the proposed method, we performed a short injection program with on-column addition of internal standard. Additionally, the method allows a simultaneous screening of non-proteinogenic amino acids that could be considered for metabolomics purposes. We design a pilot study that successfully estimated the creatinine value in 100 urine samples with (2.85 ± 1.78) mg dL -1 LOD; (8.24 ± 5.93) mg dL -1 LOQ and 82.4% accuracy. Considering that serum creatinine is also included in the clinical laboratory routines for estimated Glomerular Filtration Rate dosage, the method was complementary applied to 10 blood serum samples, which resulted in a model with (0.4 ± 0.2) mg dL -1 LOD; (2.0 ± 0.6) mg dL -1 LOQ and 83.8% of accuracy. All results were in agreement with reference values. The proposed method promotes a great analytical frequency and reproducibility with enhanced specificity compared with the ongoing protocol by Jaffe's reaction, thereby proving to be useful as an alternative for creatinine exams that might help complete a diagnosis of a series of health-related issues.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: