Salivary and serum levels of neutrophil proteases in periodontitis and rheumatoid arthritis.

Autor: Yilmaz D; Department of Periodontology, Faculty of Dentistry, Sakarya University, Sakarya, Turkey.; Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland., Niskanen K; Department of Oral and Maxillofacial Diseases, Head and Neck Center, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Gonullu E; Department of Rheumatology, Faculty of Medicine, Sakarya University, Sakarya, Turkey., Tervahartiala T; Department of Oral and Maxillofacial Diseases, Head and Neck Center, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Gürsoy UK; Department of Periodontology, Institute of Dentistry, University of Turku, Turku, Finland., Sorsa T; Department of Oral and Maxillofacial Diseases, Head and Neck Center, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.; Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
Jazyk: angličtina
Zdroj: Oral diseases [Oral Dis] 2024 Apr; Vol. 30 (3), pp. 1660-1668. Date of Electronic Publication: 2023 Apr 03.
DOI: 10.1111/odi.14574
Abstrakt: Objective: The aim was to profile serum and salivary levels of active-matrix metalloproteinase (aMMP)-8, tissue inhibitor MMP (TIMP)-1, aMMP-8/TIMP-1 ratio, total MMP (tMMP)-9, tMMP-9/TIMP-1 ratio, myeloperoxidase (MPO), and human neutrophil elastase (HNE) in periodontitis and rheumatoid arthritis (RA).
Materials and Methods: Rheumatoid arthritis patients with periodontitis (RA + P, n = 26), periodontally healthy RA patients (RA, n = 23), systemically healthy periodontitis patients (P, n = 24), and controls (C, n = 24) were included. aMMP-8 levels were determined by a time-resolved immunofluorescence assay (IFMA), TIMP-1, tMMP-9, MPO, and HNE levels were measured by enzyme-linked immunosorbent (ELISA) assays.
Results: Higher salivary aMMP-8 (p < 0.001), aMMP-8/TIMP-1 ratio (p = 0.043), tMMP-9 (p = 0.011), tMMP-9/TIMP-1 ratio (p = 0.022), MPO (p = 0.026) and HNE (p < 0.001) levels were detected in P relative to the controls. Salivary TIMP-1 was increased in RA patients regardless of periodontal status (RA + P vs. P: p = 0.038; RA vs. C: p = 0.020). Serum neutrophil proteases were increased in RA groups (RA + P, RA) compared to systemically healthy groups (P, C) (p < 0.05).
Conclusions: Serum levels of neutrophil proteases were increased in RA study groups; however rheumatologic status seemingly does not affect salivary levels of these proteins.
(© 2023 The Authors. Oral Diseases published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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