Transmembrane signaling by a synthetic receptor in artificial cells.

Autor: Søgaard AB; Department of Chemistry, Aarhus University, Aarhus C, Denmark.; iNano Interdisciplinary Nanoscience Center, Aarhus University, Aarhus C, Denmark., Pedersen AB; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Løvschall KB; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Monge P; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Jakobsen JH; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Džabbarova L; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Nielsen LF; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Stevanovic S; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Walther R; Department of Chemistry, Aarhus University, Aarhus C, Denmark., Zelikin AN; Department of Chemistry, Aarhus University, Aarhus C, Denmark. zelikin@chem.au.dk.; iNano Interdisciplinary Nanoscience Center, Aarhus University, Aarhus C, Denmark. zelikin@chem.au.dk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Mar 24; Vol. 14 (1), pp. 1646. Date of Electronic Publication: 2023 Mar 24.
DOI: 10.1038/s41467-023-37393-0
Abstrakt: Signal transduction across biological membranes is among the most important evolutionary achievements. Herein, for the design of artificial cells, we engineer fully synthetic receptors with the capacity of transmembrane signaling, using tools of chemistry. Our receptors exhibit similarity with their natural counterparts in having an exofacial ligand for signal capture, being membrane anchored, and featuring a releasable messenger molecule that performs enzyme activation as a downstream signaling event. The main difference from natural receptors is the mechanism of signal transduction, which is achieved using a self-immolative linker. The receptor scaffold is modular and can readily be re-designed to respond to diverse activation signals including biological or chemical stimuli. We demonstrate an artificial signaling cascade that achieves transmembrane enzyme activation, a hallmark of natural signaling receptors. Results of this work are relevant for engineering responsive artificial cells and interfacing them and/or biological counterparts in co-cultures.
(© 2023. The Author(s).)
Databáze: MEDLINE
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