Metabolomics of the interaction between a consortium of entomopathogenic fungi and their target insect: Mechanisms of attack and survival.

Autor: Stuart AKDC; Laboratório de Microbiologia e Biologia Molecular (LabMicro), Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil. Electronic address: andressa.katiski@gmail.com., Furuie JL; Laboratório de Microbiologia e Biologia Molecular (LabMicro), Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., Cataldi TR; Laboratório de Genética de Plantas Max Feffer, Departamento de Genética, Escola Superior de Agronomia Luiz de Queiroz - Esalq/USP, Piracicaba, São Paulo, Brazil., Stuart RM; Laboratório de Microbiologia e Biologia Molecular (LabMicro), Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., Zawadneak MAC; Laboratório de Microbiologia e Biologia Molecular (LabMicro), Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil; Programa de Pós-graduação em Agronomia Produção Vegetal, Departamento de Fitotecnia e Fitossanidade, Universidade Federal do Paraná, Curitiba, Paraná, Brazil., Labate CA; Laboratório de Genética de Plantas Max Feffer, Departamento de Genética, Escola Superior de Agronomia Luiz de Queiroz - Esalq/USP, Piracicaba, São Paulo, Brazil., Pimentel IC; Laboratório de Microbiologia e Biologia Molecular (LabMicro), Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2023 Apr; Vol. 191, pp. 105369. Date of Electronic Publication: 2023 Feb 12.
DOI: 10.1016/j.pestbp.2023.105369
Abstrakt: One of the most concerning pests that attack strawberries in Brazil is Duponchelia fovealis (Zeller), a non-native moth with no registered control methods to date. Our group recently observed that a fungal consortium formed by two strains of Beauveria bassiana (Balsamo) increased the mortality of D. fovealis more than inoculation with each strain on its own. However, the molecular interaction between the fungal consortium and the caterpillars is unknown. Thus, in this work, we sought to pioneer the evaluation of the molecular interaction between a fungal consortium of B. bassiana and D. fovealis caterpillars. We aimed to understand the biocontrol process involved in this interaction and the defense system of the caterpillar. Seven days after D. fovealis were inoculated with the consortium, the dead and surviving caterpillars were analyzed using GC-MS and LC-MS. Some of the metabolites identified in dead caterpillars have primarily antioxidant action. Other metabolites may have insecticidal potential, such as diltiazem-like and tamsulosin-like compounds, as well as 2,5-dimethoxymandelic acid. In surviving caterpillars, the main mechanisms are pro-inflammatory from 2-Palmitoylglycerol metabolite and the antifungal action of the metabolite Aegle marmelos Alkaloid-C. The metabolites identified in dead caterpillars may explain the increased mortality caused by the consortium due to its antioxidant mechanism, which can suppress the caterpillars' immune system, and insecticide action. In surviving caterpillars, the main resistance mechanisms may involve the stimulus to the immunity and antifungal action.
Competing Interests: Declaration of Competing Interest The authors declares that there is no conflict of interest.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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