Prevalence of brucellosis in livestock keepers and domestic ruminants in Baringo County, Kenya.

Autor:	Lokamar PN; Department of Public Health, School of Public Health and Community Development, Maseno University, Kisumu, Kenya.; Department of Disease Surveillance and Epidemic Response, Ministry of Health, Nairobi, Kenya., Kutwah MA; Kenya Nutritionist and Dieticians Institute, Nairobi, Kenya., Munde EO; Department of Clinical Medicine, School of Health Sciences, Kirinyaga University, Kerugoya, Kenya., Oloo D; Department of Zoology, School of Physical and Biological Sciences, Maseno University, Kisumu, Kenya., Atieli H; Department of Public Health, School of Public Health and Community Development, Maseno University, Kisumu, Kenya., Gumo S; Department of Theology and Philosophy, School of Arts and Social Sciences, Maseno University, Kisumu, Kenya., Akoko JM; Department of Biomedical Sciences and Technology, School of Public Health and Community Development, Maseno University, Kisumu, Kenya.; International Livestock Research Institute, Nairobi, Kenya., Ouma C; Department of Biomedical Sciences and Technology, School of Public Health and Community Development, Maseno University, Kisumu, Kenya.
Jazyk:	angličtina
Zdroj:	PLOS global public health [PLOS Glob Public Health] 2022 Aug 15; Vol. 2 (8), pp. e0000682. Date of Electronic Publication: 2022 Aug 15 (Print Publication: 2022).
DOI:	10.1371/journal.pgph.0000682
Abstrakt:	Brucellosis is widely distributed in more than 170 countries around the world, where it poses a huge threat to animal husbandry and human health. Brucellosis is a worldwide re-emerging zoonotic disease that poses serious public health problems in many developing countries including Kenya. However, prevalence of brucellosis has not been determined in Baringo County, Kenya, yet there is a continuous movement of cattle resulting from trade and grazing, thus predisposing many herds to brucellosis infection. We investigated the sero-prevalence of brucellosis in humans and domestic ruminants: sheep, goats, cattle and camels among livestock keeping communities in Baringo County, Kenya. In addition, we analyzed the seropositive samples for molecular detection of Brucella species. The study adopted a cross-sectional survey using quantitative data collection methods. The diagnosis was carried out using a competitive enzyme-linked immunosorbent assay (c-ELISA) and the real-time PCR assays. The sero-prevalence of brucellosis among human blood samples was 0.6% (n = 4/640) in Baringo County. About 22.30% (n = 143/640) of animal blood samples examined tested positive for Brucella genus-specific ELISA test. Cattle had a high prevalence of 22.88% (n = 93/322) followed by camels 20.00% (n = 21/105), goats 15.48% (n = 24/155) and subsequently sheep at 8.62% (n = 5/58). Overall, 7.5% (n = 6/80) of the seropositive samples amplified with the genus-specific primers. Brucella melitensis was detected in one out of the six genus positive samples, while none amplified with the B. abortus target. Even though there was high prevalence of brucellosis among livestock in Baringo County, the highest prevalence was invariably noted in cattle, followed by camels, goats and sheep, respectively. Livestock keepers had low prevalence of brucellosis. This implies that there was low risk of transmission of brucellosis between livestock keepers and their livestock. Competing Interests: The authors have declared that no competing interests exist. (Copyright: © 2022 Lokamar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze:	MEDLINE
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