The lignin-degrading abilities of Gelatoporia subvermispora gat1 and pex1 mutants generated via CRISPR/Cas9.

Autor: Nakazawa T; Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan., Inoue C; Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan., Morimoto R; Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan., Nguyen DX; Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan., Bao D; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China., Kawauchi M; Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan., Sakamoto M; Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan., Honda Y; Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
Jazyk: angličtina
Zdroj: Environmental microbiology [Environ Microbiol] 2023 Aug; Vol. 25 (8), pp. 1393-1408. Date of Electronic Publication: 2023 Mar 23.
DOI: 10.1111/1462-2920.16372
Abstrakt: White-rot fungi efficiently degrade wood lignin; however, the mechanisms involved remain largely unknown. Recently, a forward genetics approach to identify several genes in Pleurotus ostreatus (Agaricales) in which mutations cause defects in wood lignin degradation was used. For example, pex1 encodes a peroxisome biogenesis factor and gat1 encodes a putative Agaricomycetes-specific DNA-binding transcription factor. In this study, we examined the effects of single-gene mutations in pex1 or gat1 on wood lignin degradation in another white-rot fungus, Gelatoporia (Ceriporiopsis) subvermispora (Polyporales), to investigate conserved and derived degradation mechanisms in white-rot fungi. G. subvermispora pex1 and gat1 single-gene mutant strains were generated from a monokaryotic wild-type strain, FP-90031-Sp/1, using plasmid-based CRISPR/Cas9. As in P. ostreatus, Gsgat1 mutants were nearly unable to degrade lignin sourced from beech wood sawdust medium (BWS), while Gspex1 mutants exhibited a delay in lignin degradation. We also found that the transcripts of lignin-modifying enzyme-encoding genes, mnp4, mnp5, mnp6, mnp7, and mnp11, which predominantly accumulate in FP-90031-Sp/1 cultured with BWS, were greatly downregulated in Gsgat1 mutants. Taken together, the results suggest that Gat1 may be a conserved regulator of the ligninolytic system of white-rot fungi and that the contribution of peroxisomes to the ligninolytic system may differ among species.
(© 2023 Applied Microbiology International and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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