Cryptosporidium uses multiple distinct secretory organelles to interact with and modify its host cell.

Autor:	Guérin A; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Strelau KM; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Barylyuk K; Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK., Wallbank BA; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA., Berry L; LPHI, CNRS, Université de Montpellier, Montpellier 34095, France., Crook OM; Department of Statistics, University of Oxford, Oxford OX1 3LB, UK., Lilley KS; Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK., Waller RF; Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK., Striepen B; Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: striepen@upenn.edu.
Jazyk:	angličtina
Zdroj:	Cell host & microbe [Cell Host Microbe] 2023 Apr 12; Vol. 31 (4), pp. 650-664.e6. Date of Electronic Publication: 2023 Mar 22.
DOI:	10.1016/j.chom.2023.03.001
Abstrakt:	Cryptosporidium is a leading cause of diarrheal disease in children and an important contributor to early childhood mortality. The parasite invades and extensively remodels intestinal epithelial cells, building an elaborate interface structure. How this occurs at the molecular level and the contributing parasite factors are largely unknown. Here, we generated a whole-cell spatial proteome of the Cryptosporidium sporozoite and used genetic and cell biological experimentation to discover the Cryptosporidium-secreted effector proteome. These findings reveal multiple organelles, including an original secretory organelle, and generate numerous compartment markers by tagging native gene loci. We show that secreted proteins are delivered to the parasite-host interface, where they assemble into different structures including a ring that anchors the parasite into its unique epicellular niche. Cryptosporidium thus uses a complex set of secretion systems during and following invasion that act in concert to subjugate its host cell. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023. Published by Elsevier Inc.)
Databáze:	MEDLINE
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