Embryotoxicity of silica nanoparticles in the drug delivery of domperidone in zebrafish.

Autor: Valadares LPA; Laboratory of Tropical Fish Biotechnology and Physiology at the Federal University of Jataí, Jataí, Goiás, Brazil., Lima LCO; Aquam production and conservation of aquatic species biodiversity, Federal University of Rio Grande do Sul, Porto Alegra, Rio Grande do Sul, Brazil., Saboia-Morais SMT; Cellular Behavior Laboratory, Federal University of Goiás, Goiânia, Goiás, Brazil., Arantes TM; Chemistry Department at the Federal University of Jataí, Jataí, Goiás, Brazil., Cristovan FH; Chemistry Department at the Federal University of Jataí, Jataí, Goiás, Brazil., da Silva NM; Chemistry Department at the Federal University of Jataí, Jataí, Goiás, Brazil., Andrade AB; Chemistry Department at the Federal University of Jataí, Jataí, Goiás, Brazil., Ribeiro SAB; Department biological sciences at the Federal University of Jataí, Jataí, Goiás, Brazil., Alves BG; Invited Professor of the Postgraduate Program in Animal Bioscience at the Federal University of Jataí, Jataí, Goiás Brazil., Virote BDCR; Center for Studies in Freshwater Fish Physiology at the Federal University of Lavras, Lavras, Minas Gerais, Brazil., da Silva IC; Department of Pharmacology, University of São Paulo - ICB/USP, São Paulo - Capital, São Paulo, Brazil., Machado MRF; Laboratory of Tropical Fish Biotechnology and Physiology at the Federal University of Jataí, Jataí, Goiás, Brazil. Electronic address: monica_rodrigues@ufj.edu.br.
Jazyk: angličtina
Zdroj: Aquatic toxicology (Amsterdam, Netherlands) [Aquat Toxicol] 2023 May; Vol. 258, pp. 106454. Date of Electronic Publication: 2023 Feb 24.
DOI: 10.1016/j.aquatox.2023.106454
Abstrakt: Domperidone is a dopamine D2 receptor inhibitor that stimulates pituitary gonadotropins. It is usually associated with synthetic GnRHa to promote spawning in fish. However, the route of administration used, intramuscular injection, can be quite stressful. Little is known about the effects of domperidone, as well as other routes. This study aims to evaluate the toxicity of domperidone encapsulated by silica nanoparticles in zebrafish embryos. The study involved four groups with three concentrations: 1. domperidone (DP) 0.0001, 0.0002 and 0.0004 mg/mL; 2. DP associated with silica nanoparticles (SiNPs) 0.0001 + 1.1, 0.0002 + 2.2 and 0.0004 + 4.4 mg/mL; 3. SiNPs 1.1, 2.2 and 4.4 mg/mL and 4. Control (E3), with four repetitions per group. Survival, teratogen and heart rate (HR) were evaluated over a period of 168 hpf. Survival was higher in DP + SiNPs treatment, HR was lower in treatment with 4.4 mg/mL of SiNPs, while treatment with 0.004 mg/mL of DP increased HR. This study demonstrated that the association of DP and SiNPs decreased the toxicity of both DP and SiNPs, demonstrating that this may be a viable alternative to reduce the possible cardiotoxic effects of DP.
Competing Interests: Declaration of Competing Interest I, Monica Rodrigues Ferreira Machado, professor at the Federal University of Jataí, declare for all due purposes that this work has not been submitted to other journals and that its content has not been presented in other works. Lorranny Pereira de Assis Valadares reports financial support was provided by Fundação de amparo à pesquisa do estado de Goiás.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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