Photorhabdus lux- operon heat shock-like regulation.

Autor:	Fomin VV; Laboratory of Molecular Genetics, Moscow Institute of Physics and Technology, Institutsky Lane 9, Dolgoprudny, Moscow Region, 141700, Russian Federation.; Laboratory of Microbiology, BIOTECH University, Volokolamskoe Highway 11, Moscow 125080, Russian Federation., Bazhenov SV; Laboratory of Molecular Genetics, Moscow Institute of Physics and Technology, Institutsky Lane 9, Dolgoprudny, Moscow Region, 141700, Russian Federation., Kononchuk OV; Laboratory of Molecular Genetics, Moscow Institute of Physics and Technology, Institutsky Lane 9, Dolgoprudny, Moscow Region, 141700, Russian Federation.; Laboratory of Microbiology, BIOTECH University, Volokolamskoe Highway 11, Moscow 125080, Russian Federation., Matveeva VO; Laboratory of Molecular Genetics, Moscow Institute of Physics and Technology, Institutsky Lane 9, Dolgoprudny, Moscow Region, 141700, Russian Federation., Zarubina AP; Biological Faculty, Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 119992, Russian Federation., Spiridonov SE; Centre of Parasitology, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii Prospect, 33, Moscow, 119071, Russian Federation., Manukhov IV; Laboratory of Molecular Genetics, Moscow Institute of Physics and Technology, Institutsky Lane 9, Dolgoprudny, Moscow Region, 141700, Russian Federation.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Mar 11; Vol. 9 (3), pp. e14527. Date of Electronic Publication: 2023 Mar 11 (Print Publication: 2023).
DOI:	10.1016/j.heliyon.2023.e14527
Abstrakt:	For decades, transcription of Photorhabdus luminescens lux -operon was considered being constitutive. Therefore, this lux -operon has been used for measurements in non-specific bacterial luminescent biosensors. Here, the expression of Photorhabdus lux -operon under high temperature was studied. The expression was researched in the natural strain Photorhabdus temperata and in the heterologous system of Escherichia coli . P. temperata FV2201 bacterium was isolated from soil in the Moscow region (growth optimum 28 °C). We showed that its luminescence significantly increases when the temperature rises to 34 °C. The increase in luminescence is associated with an increase in the transcription of luxCDABE genes, which was confirmed by RT-PCR. The promoter of the lux -operon of the related bacterium P. luminescens ZM1 from the forests of Moldova, being cloned in the heterologous system of E. coli , is activated when the temperature rises from room temperature to 42 °C. When heat shock is caused by ethanol addition, transcription of lux -operon increases only in the natural strain of P. temperata , but not in the heterologous system of E. coli cells. In addition, the activation of the lux -operon of P. luminescens persists in E. coli strains deficient in both the rpoH and rpoE genes. These results indicate the presence of sigma 32 and sigma 24 independent heat-shock-like mechanism of regulation of the lux -operon of P. luminescens in the heterologous E. coli system. Competing Interests: The authors declare no conflict of interest. (© 2023 The Authors.)
Databáze:	MEDLINE
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