Metagenomic insights into the plasma virome of Brazilian patients with prostate cancer.

Autor: Luciola Zanette D; Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Professor Algacyr Munhoz Mader, Curitiba, Parana, Brazil., Andrade Coelho KBC; Department of Anatomy and Surgery, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirao Preto, Sao Paulo, Brazil., de Carvalho E; Butantan Institute, São Paulo, São Paulo, Brazil., Aoki MN; Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Professor Algacyr Munhoz Mader, Curitiba, Parana, Brazil., Nardin JM; Department of Urology, Erasto Gaertner Hospital, Curitiba, Parana, Brazil., Araújo Lalli L; Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (FIOCRUZ), Professor Algacyr Munhoz Mader, Curitiba, Parana, Brazil., Dos Santos Bezerra R; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Giovanetti M; Department of Science and Technology for Humans and the Environment, University of Campus Bio-Medico di Roma, Rome, Italy.; Laboratory of Flaviviruses, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil., Simionatto Zucherato V; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Montenegro de Campos G; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., de Souza Todão Bernardino J; Butantan Institute, São Paulo, São Paulo, Brazil., Louis Viala V; Butantan Institute, São Paulo, São Paulo, Brazil., Ciccozzi M; Epidemiology and Statistic Unit, University of Campus Bio-Medico di Roma, Rome, Italy., Junior Alcantara LC; Laboratory of Flaviviruses, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, Brazil., Coccuzzo Sampaio S; Butantan Institute, São Paulo, São Paulo, Brazil., Elias MC; Butantan Institute, São Paulo, São Paulo, Brazil., Kashima S; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Tadeu Covas D; Butantan Institute, São Paulo, São Paulo, Brazil.; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil., Nanev Slavov S; Butantan Institute, São Paulo, São Paulo, Brazil.; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: Molecular & cellular oncology [Mol Cell Oncol] 2023 Mar 15; Vol. 10 (1), pp. 2188858. Date of Electronic Publication: 2023 Mar 15 (Print Publication: 2023).
DOI: 10.1080/23723556.2023.2188858
Abstrakt: Growing evidence suggests that metavirome changes could be associated increased risk for malignant cell transformation. Considering Viruses have been proposed as factors for prostate cancer induction. The objective of this study was to examine the composition of the plasma metavirome of patients with prostate cancer. Blood samples were obtained from 49 male patients with primary prostate adenocarcinoma. Thirty blood donors were included as a control group. The obtained next-generation sequencing data were analyzed using a bioinformatic pipeline for virus metagenomics. Viral reads with higher abundance were assembled in contigs and analyzed taxonomically. Viral agents of interest were also confirmed by qPCR. Anelloviruses and the Human Pegivirus-1 (HPgV-1) were the most abundant component of plasma metavirome. Clinically important viruses like hepatitis C virus (HCV), cytomegalovirus and human adenovirus type C were also identified. In comparison, the blood donor virome was exclusively composed of torque teno virus types (TTV) types. The performed HPgV-1 and HCV phylogeny revealed that these viruses belong to commonly detected in Brazil genotypes. Our study sheds light on the plasma viral abundance in patients with prostatic cancer. The obtained viral diversity allowed us to separate the patients and controls, probably suggesting that malignant processes may influence virome composition. More complex and multiple approach investigations are necessary to examine the likely causal relationship between metavirome and its nvolvement in prostate cancer.
Competing Interests: No potential conflict of interest was reported by the authors.
(© 2023 The Author(s). Published with license by Taylor & Francis Group, LLC.)
Databáze: MEDLINE
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