A Pilot Study Exploring Temporal Development of Gut Microbiome/Metabolome in Breastfed Neonates during the First Week of Life.

Autor:	Awan I; Department of Medicine, Loma Linda University Medical Center, Loma Linda, CA, USA., Schultz E; Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA., Sterrett JD; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, USA., Dawud LM; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, USA., Kessler LR; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, USA., Schoch D; Cooper Medical School of Rowan University and Cooper University Hospital, Camden, NJ, USA., Lowry CA; Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, USA., Feldman-Winter L; Cooper Medical School of Rowan University and Cooper University Hospital, Camden, NJ, USA., Phadtare S; Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ, USA.
Jazyk:	angličtina
Zdroj:	Pediatric gastroenterology, hepatology & nutrition [Pediatr Gastroenterol Hepatol Nutr] 2023 Mar; Vol. 26 (2), pp. 99-115. Date of Electronic Publication: 2023 Mar 07.
DOI:	10.5223/pghn.2023.26.2.99
Abstrakt:	Purpose: Exclusive breastfeeding promotes gut microbial compositions associated with lower rates of metabolic and autoimmune diseases. Its cessation is implicated in increased microbiome-metabolome discordance, suggesting a vulnerability to dietary changes. Formula supplementation is common within our low-income, ethnic-minority community. We studied exclusively breastfed (EBF) neonates' early microbiome-metabolome coupling in efforts to build foundational knowledge needed to target this inequality. Methods: Maternal surveys and stool samples from seven EBF neonates at first transitional stool (0-24 hours), discharge (30-48 hours), and at first appointment (days 3-5) were collected. Survey included demographics, feeding method, medications, medical history and tobacco and alcohol use. Stool samples were processed for 16S rRNA gene sequencing and lipid analysis by gas chromatography-mass spectrometry. Alpha and beta diversity analyses and Procrustes randomization for associations were carried out. Results: Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria were the most abundant taxa. Variation in microbiome composition was greater between individuals than within ( p =0.001). Palmitic, oleic, stearic, and linoleic acids were the most abundant lipids. Variation in lipid composition was greater between individuals than within ( p =0.040). Multivariate composition of the metabolome, but not microbiome, correlated with time ( p =0.030). Total lipids, saturated lipids, and unsaturated lipids concentrations increased over time ( p =0.012, p =0.008, p =0.023). Alpha diversity did not correlate with time ( p =0.403). Microbiome composition was not associated with each samples' metabolome ( p =0.450). Conclusion: Neonate gut microbiomes were unique to each neonate; respective metabolome profiles demonstrated generalizable temporal developments. The overall variability suggests potential interplay between influences including maternal breastmilk composition, amount consumed and living environment. Competing Interests: Conflict of Interest: The authors have no financial conflicts of interest. (Copyright © 2023 by The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition.)
Databáze:	MEDLINE
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