Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network.

Autor: De Giglio O; Interdisciplinary Department of Medicine, Section of Hygiene, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124, Bari, Italy. osvalda.degiglio@uniba.it., D'Ambrosio M; Department of Biomedical Sciences and Human Oncology, Section of Hygiene, Medical School, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124, Bari, Italy., Spagnuolo V; Department of Biomedical Sciences and Human Oncology, Section of Hygiene, Medical School, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124, Bari, Italy., Diella G; Interdisciplinary Department of Medicine, Section of Hygiene, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124, Bari, Italy., Fasano F; Interdisciplinary Department of Medicine, Section of Hygiene, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124, Bari, Italy., Leone CM; Section Hygiene - AOU Policlinico of Bari, Piazza G. Cesare 11, 70124, Bari, Italy., Lopuzzo M; Department of Biomedical Sciences and Human Oncology, Section of Hygiene, Medical School, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124, Bari, Italy., Trallo V; Section Hygiene - AOU Policlinico of Bari, Piazza G. Cesare 11, 70124, Bari, Italy., Calia C; Department of Biology, University of Bari Aldo Moro, Via Orabona 4, 70126, Bari, Italy., Oliva M; Department of Biology, University of Bari Aldo Moro, Via Orabona 4, 70126, Bari, Italy., Pazzani C; Department of Biology, University of Bari Aldo Moro, Via Orabona 4, 70126, Bari, Italy., Iacumin L; Department of Agricultural, Food, Environmental and Animal Science, University of Udine, Via Sondrio 2/a, 33100, Udine, Italy., Barigelli S; Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06122, Perugia, Italy., Petricciuolo M; Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06122, Perugia, Italy., Federici E; Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06122, Perugia, Italy., Lisena FP; Health Management, A.O.U. Policlinico of Bari, Piazza G. Cesare 11, 70124, Bari, Italy., Minicucci AM; Health Management, A.O.U. Policlinico of Bari, Piazza G. Cesare 11, 70124, Bari, Italy., Montagna MT; Regional Reference Laboratory of Clinical and Environmental Surveillance of Legionellosis, Interdisciplinary Department of Medicine, Section of Hygiene, University of Bari Aldo Moro, Piazza G. Cesare 11, 70124, Bari, Italy.
Jazyk: angličtina
Zdroj: Environmental monitoring and assessment [Environ Monit Assess] 2023 Mar 22; Vol. 195 (4), pp. 496. Date of Electronic Publication: 2023 Mar 22.
DOI: 10.1007/s10661-023-11078-z
Abstrakt: Understanding the actual distribution of different Legionella species in water networks would help prevent outbreaks. Culture investigations followed by serological agglutination tests, with poly/monovalent antisera, still represent the gold standard for isolation and identification of Legionella strains. However, also MALDI-TOF and mip-gene sequencing are currently used. This study was conducted to genetically correlate strains of Legionella non pneumophila (L-np) isolated during environmental surveillance comparing different molecular techniques. Overall, 346 water samples were collected from the water system of four pavilions located in a hospital of the Apulia Region of Italy. Strains isolated from the samples were then identified by serological tests, MALDI-TOF, and mip-gene sequencing. Overall, 24.9% of water samples were positive for Legionella, among which the majority were Legionella pneumophila (Lpn) 1 (52.3%), followed by Lpn2-15 (20.9%), L-np (17.4%), Lpn1 + Lpn2-15 (7.1%), and L-np + Lpn1 (2.3%). Initially, L-np strains were identified as L. bozemanii by monovalent antiserum, while MALDI-TOF and mip-gene sequencing assigned them to L. anisa. More cold water than hot water samples were contaminated by L. anisa (p < 0.001). PFGE, RAPD, Rep-PCR, and SAU-PCR were performed to correlate L. anisa strains. Eleven out of 14 strains identified in all four pavilions showed 100% of similarity upon PFGE analysis. RAPD, Rep-PCR, and SAU-PCR showed greater discriminative power than PFGE.
(© 2023. The Author(s).)
Databáze: MEDLINE
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