Quantitative videomicroscopy reveals latent control of cell-pair rotations in vivo.

Autor:	Kozak EL; Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München, Munich D-85764, Germany., Miranda-Rodríguez JR; Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München, Munich D-85764, Germany., Borges A; Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München, Munich D-85764, Germany.; Systems Biology Group (SysBio), Institute of Physics of Liquids and Biological Systems (IFLySIB), National Scientific and Technical Research Council (CONICET), University of La Plata, La Plata B1900BTE, Argentina.; Graduate School of Quantitative Biosciences, Ludwig Maximilian University, Munich 81377, Germany., Dierkes K; Centre for Genomic Regulation, Barcelona 08003, Spain., Mineo A; Centre for Genomic Regulation, Barcelona 08003, Spain., Pinto-Teixeira F; Centre for Genomic Regulation, Barcelona 08003, Spain., Viader-Llargués O; Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München, Munich D-85764, Germany., Solon J; Centre for Genomic Regulation, Barcelona 08003, Spain.; Instituto Biofisika, Basque Excellence Research Centre, Leioa 48940, Spain., Chara O; Systems Biology Group (SysBio), Institute of Physics of Liquids and Biological Systems (IFLySIB), National Scientific and Technical Research Council (CONICET), University of La Plata, La Plata B1900BTE, Argentina.; School of Biosciences, University of Nottingham, Sutton Bonington Campus, Nottingham LE12 5RD, UK.; Instituto de Tecnología, Universidad Argentina de la Empresa, Buenos Aires C1073AAO, Argentina., López-Schier H; Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München, Munich D-85764, Germany.; Graduate School of Quantitative Biosciences, Ludwig Maximilian University, Munich 81377, Germany.; Division of Science, New York University Abu Dhabi, Saadiyat Island 129188, United Arab Emirates.
Jazyk:	angličtina
Zdroj:	Development (Cambridge, England) [Development] 2023 May 01; Vol. 150 (9). Date of Electronic Publication: 2023 May 03.
DOI:	10.1242/dev.200975
Abstrakt:	Collective cell rotations are widely used during animal organogenesis. Theoretical and in vitro studies have conceptualized rotating cells as identical rigid-point objects that stochastically break symmetry to move monotonously and perpetually within an inert environment. However, it is unclear whether this notion can be extrapolated to a natural context, where rotations are ephemeral and heterogeneous cellular cohorts interact with an active epithelium. In zebrafish neuromasts, nascent sibling hair cells invert positions by rotating ≤180° around their geometric center after acquiring different identities via Notch1a-mediated asymmetric repression of Emx2. Here, we show that this multicellular rotation is a three-phasic movement that progresses via coherent homotypic coupling and heterotypic junction remodeling. We found no correlation between rotations and epithelium-wide cellular flow or anisotropic resistive forces. Moreover, the Notch/Emx2 status of the cell dyad does not determine asymmetric interactions with the surrounding epithelium. Aided by computer modeling, we suggest that initial stochastic inhomogeneities generate a metastable state that poises cells to move and spontaneous intercellular coordination of the resulting instabilities enables persistently directional rotations, whereas Notch1a-determined symmetry breaking buffers rotational noise. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2023. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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