Decreased circulating microRNA-21 and microRNA-143 are associated to pulmonary hypertension.

Autor: Düzgün Z; Department of Medical Biology, Faculty of Medicine, Giresun University, Giresun, Turkey., Kayıkçıoğlu LM; Department of Cardiology, Faculty of Medicine, Ege University, İzmir, Turkey., Aktan Ç; Department of Medical Biology, Beykent University School of Medicine, İstanbul, Turkey., Bara B; Department of Medical Biology, Faculty of Medicine, Ege University, İzmir, Turkey., Eroğlu FZ; Department of Medical Biology, Faculty of Medicine, Ege University, İzmir, Turkey., Yağmur B; Department of Cardiology, Faculty of Medicine, Ege University, İzmir, Turkey., Çetintaş VB; Department of Medical Biology, Faculty of Medicine, Ege University, İzmir, Turkey., Bayındır M; Department of Medical Biology, Faculty of Medicine, Ege University, İzmir, Turkey., Nalbantgil S; Department of Cardiology, Faculty of Medicine, Ege University, İzmir, Turkey., Vardarli AT; Department of Medical Biology, Faculty of Medicine, Ege University, İzmir, Turkey.
Jazyk: angličtina
Zdroj: Turkish journal of medical sciences [Turk J Med Sci] 2023 Feb; Vol. 53 (1), pp. 130-141. Date of Electronic Publication: 2023 Feb 22.
DOI: 10.55730/1300-0144.5566
Abstrakt: Background: Pulmonary arterial hypertension (PAH) is characterized by maladaptation of pulmonary vasculature which is leading to right ventricular hypertrophy and heart failure. miRNAs play a crucial role in the regulation of many diseases such as viral infection, cancer, cardiovascular diseases, and pulmonary hypertension (PH). In this study, we aimed to investigate the expression pattern of eight human plasma miRNAs (hsa-miR-21-3p, hsa-miR-143- 3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsamiR-206, hsa-miR-210-3p) in mild-to-severe PH patients and healthy controls.
Methods: : miRNAs were extracted from the peripheral plasma of the PH patients (n: 44) and healthy individuals (n: 30) by using the miRNA Isolation Kit. cDNA was synthesized using All in-One First strand cDNA Synthesis Kit. Expression of the human plasma hsa-miR- 21-3p, hsa-miR-143-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204- 3p, hsa-miR-206, hsa-miR210-3p, and miRNAs were analyzed by qRT-PCR.
Results: According to our results, in PH patients hsa-miR-21-3p and hsa-miR-143-3p expression levels were decreased by 4.7 and 2.3 times, respectively. No significant changes were detected in hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-190a, hsa-miR-204-3p, hsamiR-206, and hsa-miR-210-3p expression levels between PH and control groups. In addition, considering the severity of the disease, it was observed that the decrease in miR-138, miR-143, miR-145, miR-190, mir-204, mir-206 and miR-208 expressions was significant in patients with severe PH.
Discussion: : In the early diagnosis of PAH, hsa-miR-21-3p and especially hsa-miR-143-3p in peripheral plasma can be considered as potential biomarkers.
Databáze: MEDLINE
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