An expanded GCaMP reporter toolkit for functional imaging in C. elegans .

Autor: Ding J; These authors contributed equally.; Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA., Peng L; These authors contributed equally.; Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA., Moon S; These authors contributed equally.; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA., Lee HJ; These authors contributed equally.; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA., Patel DS; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.; Present address: NemaLife Inc, Lubbock, TX 79409, USA., Lu H; Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.; School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2023 Mar 08. Date of Electronic Publication: 2023 Mar 08.
DOI: 10.1101/2023.03.06.531342
Abstrakt: In living organisms, changes in calcium flux are integral to many different cellular functions and are especially critical for the activity of neurons and myocytes. Genetically encoded calcium indicators (GECIs) have been popular tools for reporting changes in calcium levels in vivo . In particular, GCaMP, derived from GFP, are the most widely used GECIs and have become an invaluable toolkit for neurophysiological studies. Recently, new variants of GCaMP, which offer a greater variety of temporal dynamics and improved brightness, have been developed. However, these variants are not readily available to the Caenorhabditis elegans research community. This work reports a set of GCaMP6 and jGCaMP7 reporters optimized for C. elegans studies. Our toolkit provides reporters with improved dynamic range, varied kinetics, and targeted subcellular localizations. Besides optimized routine uses, this set of reporters are also well-suited for studies requiring fast imaging speeds and low magnification or low-cost platforms.
Databáze: MEDLINE