Predatory fireflies and their toxic firefly prey have evolved distinct toxin resistance strategies.

Autor:	Yang L; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, USA., Borne F; Department of Biological Sciences, Columbia University, New York, USA., Betz A; Department of Applied Entomology, University of Hohenheim, Stuttgart, Germany., Aardema ML; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, USA.; Department of Biology, Montclair State University, Montclair, USA., Zhen Y; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, USA.; School of Life Sciences, Westlake University, Hangzhou, China., Peng J; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, USA., Visconti R; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, USA., Wu M; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, USA., Roland BP; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, USA.; Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh School of Medicine, Pittsburgh, USA., Talsma AD; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, USA.; Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh School of Medicine, Pittsburgh, USA., Palladino MJ; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, USA.; Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh School of Medicine, Pittsburgh, USA., Petschenka G; Department of Applied Entomology, University of Hohenheim, Stuttgart, Germany., Andolfatto P; Department of Biological Sciences, Columbia University, New York, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Oct 24. Date of Electronic Publication: 2023 Oct 24.
DOI:	10.1101/2023.03.08.531760
Abstrakt:	Toxic cardiotonic steroids (CTS) act as a defense mechanism in many firefly species (Lampyridae) by inhibiting a crucial enzyme called Na + ,K + -ATPase (NKA). While most fireflies produce these toxins internally, species of the genus Photuris acquire them from a surprising source: predation on other fireflies. The contrasting physiology of toxin exposure and sequestration between Photuris and other firefly genera suggests that distinct strategies may be required to prevent self-intoxication. Our study demonstrates that both Photuris and their firefly prey have evolved highly-resistant NKAs. Using an evolutionary analysis of the specific target of CTS (ATPα) in fireflies, and gene-editing in Drosophila , we find that the initial steps towards resistance were shared among Photuris and other firefly lineages. However, the Photuris lineage subsequently underwent multiple rounds of gene duplication and neofunctionalization, resulting in the development of ATPα paralogs that are differentially expressed and exhibit increasing resistance to CTS. In contrast, other firefly species have maintained a single copy. Our results implicate gene duplication as a facilitator in the transition of Photuris to its distinct ecological role as predator of toxic firefly prey. Competing Interests: Declaration of Interests The authors declare no competing interests.
Databáze:	MEDLINE
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