Large-scale morphometry of the subarachnoid space of the optic nerve.

Autor: Rossinelli D; Institute of Neuroradiology, Kantonsspital Aarau, Tellstrasse 25, CH-5001, Aarau, Switzerland. rossinelli.diego@gmail.com.; Institute of Physiology, University of Zurich, Zurich, Switzerland. rossinelli.diego@gmail.com., Killer HE; Department of Biomedicine, University of Basel, Basel, Switzerland., Meyer P; Ocular Pharmacology and Physiology, University Hospital of Basel, Basel, Switzerland., Knott G; Biological Electron Microscopy Facility (BioEM), Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland., Fourestey G; Scientific IT & Application Support (SCITAS), Swiss Federal Institute of Technology Lausanne (EPFL), Lausanne, Switzerland., Kurtcuoglu V; Institute of Physiology, University of Zurich, Zurich, Switzerland., Kohler C; Department of Biomedicine, University of Basel, Basel, Switzerland., Gruber P; Institute of Neuroradiology, Kantonsspital Aarau, Tellstrasse 25, CH-5001, Aarau, Switzerland., Remonda L; Institute of Neuroradiology, Kantonsspital Aarau, Tellstrasse 25, CH-5001, Aarau, Switzerland.; Medical Faculty, University of Bern, Bern, Switzerland., Neutzner A; Department of Biomedicine, University of Basel, Basel, Switzerland., Berberat J; Institute of Neuroradiology, Kantonsspital Aarau, Tellstrasse 25, CH-5001, Aarau, Switzerland.; Geriatric Psychiatry, Department of Psychiatry, University Hospitals of Geneva, University of Geneva, Geneva, Switzerland.
Jazyk: angličtina
Zdroj: Fluids and barriers of the CNS [Fluids Barriers CNS] 2023 Mar 21; Vol. 20 (1), pp. 21. Date of Electronic Publication: 2023 Mar 21.
DOI: 10.1186/s12987-023-00423-6
Abstrakt: Background: The meninges, formed by dura, arachnoid and pia mater, cover the central nervous system and provide important barrier functions. Located between arachnoid and pia mater, the cerebrospinal fluid (CSF)-filled subarachnoid space (SAS) features a variety of trabeculae, septae and pillars. Like the arachnoid and the pia mater, these structures are covered with leptomeningeal or meningothelial cells (MECs) that form a barrier between CSF and the parenchyma of the optic nerve (ON). MECs contribute to the CSF proteome through extensive protein secretion. In vitro, they were shown to phagocytose potentially toxic proteins, such as α-synuclein and amyloid beta, as well as apoptotic cell bodies. They therefore may contribute to CSF homeostasis in the SAS as a functional exchange surface. Determining the total area of the SAS covered by these cells that are in direct contact with CSF is thus important for estimating their potential contribution to CSF homeostasis.
Methods: Using synchrotron radiation-based micro-computed tomography (SRµCT), two 0.75 mm-thick sections of a human optic nerve were acquired at a resolution of 0.325 µm/pixel, producing images of multiple terabytes capturing the geometrical details of the CSF space. Special-purpose supercomputing techniques were employed to obtain a pixel-accurate morphometric description of the trabeculae and estimate internal volume and surface area of the ON SAS.
Results: In the bulbar segment, the ON SAS microstructure is shown to amplify the MECs surface area up to 4.85-fold compared to an "empty" ON SAS, while just occupying 35% of the volume. In the intraorbital segment, the microstructure occupies 35% of the volume and amplifies the ON SAS area 3.24-fold.
Conclusions: We provided for the first time an estimation of the interface area between CSF and MECs. This area is of importance for estimating a potential contribution of MECs on CSF homeostasis.
(© 2023. The Author(s).)
Databáze: MEDLINE
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