Toward an experimental system for the examination of protein mannosylation in Actinobacteria.

Autor:	Saxena H; Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada., Buenbrazo N; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada., Song WY; Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada., Li C; Department of Medical Genetics, University of Toronto, Toronto, ON M5R 0A3, Canada., Brochu D; Human Health Therapeutics, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada., Robotham A; Human Health Therapeutics, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada., Ding W; Human Health Therapeutics, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada., Tessier L; Human Health Therapeutics, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada., Chen R; Human Health Therapeutics, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada., Kelly J; Human Health Therapeutics, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada., Wakarchuk W; Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada.
Jazyk:	angličtina
Zdroj:	Glycobiology [Glycobiology] 2023 Jun 21; Vol. 33 (6), pp. 512-524.
DOI:	10.1093/glycob/cwad023
Abstrakt:	The Actinobacterial species Cellulomonas fimi ATCC484 has long been known to secrete mannose-containing proteins, but a closer examination of glycoproteins associated with the cell has never been reported. Using ConA lectin chromatography and mass spectrometry, we have surveyed the cell-associated glycoproteome from C. fimi and collected detailed information on the glycosylation sites of 19 cell-associated glycoproteins. In addition, we have expressed a previously known C. fimi secreted cellulase, Celf_3184 (formerly CenA), a putative peptide prolyl-isomerase, Celf_2022, and a penicillin-binding protein, Celf_0189, in the mannosylation capable host, Corynebacterium glutamicum. We found that the glycosylation machinery in C. glutamicum was able to use the recombinant C. fimi proteins as substrates and that the glycosylation matched closely that found in the native proteins when expressed in C. fimi. We are pursuing this observation as a prelude to dissecting the biosynthetic machinery and biological consequences of this protein mannosylation. (© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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