Detection of antimicrobial resistance genes in Lactobacillus spp. from poultry probiotic products and their horizontal transfer among Escherichia coli .

Autor:	Rokon-Uz-Zaman M; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Bushra A; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Pospo TA; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Runa MA; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Tasnuva S; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Parvin MS; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh., Islam MT; Population Medicine and AMR Laboratory, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Jazyk:	angličtina
Zdroj:	Veterinary and animal science [Vet Anim Sci] 2023 Mar 10; Vol. 20, pp. 100292. Date of Electronic Publication: 2023 Mar 10 (Print Publication: 2023).
DOI:	10.1016/j.vas.2023.100292
Abstrakt:	The study was conducted to identify the antimicrobial resistance genes (ARGs) in Lactobacillus spp. from poultry probiotic products and their potential to spread among Escherichia coli. Lactobacillus spp. were isolated and identified from 35 poultry probiotic samples based on the cultural, biochemical, and molecular findings. All the isolates ( n  = 35) were screened for the presence of some ARGs such as β-lactamases encoding genes ( bla TEM, bla CTXM-1, and bla CTXM-2), plasmid-mediated quinolone resistance gene ( qnr A, qnr B, and qnr S), and tetracycline resistance genes ( tet A and tet B). Five Lactobacillus spp. isolates from three brands were positive for one or more ARGs. The qnr S was detected in four isolates. The bla TEM and tet B were detected in two isolates. One isolate contained bla CTX-M-1, bla CTX-M-2, and tet A genes. Brand-wise analysis revealed that one isolate from Brand 4 contained bla TEM, bla CTX-M-1, bla CTX-M-2, qnr S, and tet A genes, one isolate from Brand 2 contained bla TEM gene, and three isolates from Brand 7 harbored qnr S gene. The co-culture of Lactobacillus spp. and E. coli resulted in the transmission of qnr S, CTX-M-1, and tet A from Lactobacillus spp. to E. coli . Results of antimicrobial susceptibility test revealed that the highest resistance was observed to cefepime and cefotaxime followed by penicillin G, oxacillin, cefuroxime, and ofloxacin. The findings of the present study indicate the potential risk of horizontal spread of antimicrobial resistance through probiotic bacteria among the poultry population. Therefore, it is very necessary to check for ARGs along with other attributes of probiotic bacteria to avoid the inclusion of resistant strains in probiotics. Competing Interests: The authors declare that they have no known competing interests. (© 2023 The Author(s).)
Databáze:	MEDLINE
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