Neoehrlichia mikurensis in Danish immunocompromised patients: a retrospective cohort study.

Autor: Gynthersen RMM; Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Blegdamsvej 9, 2100, Copenhagen, Denmark. rosa.maja.moehring.gynthersen.01@regionh.dk., Hansen MF; Department of Science and Environment, Roskilde University, Universitetsvej 1, 4000, Roskilde, Denmark. mefrha@ruc.dk., Ocias LF; Department of Clinical Microbiology, Karlstad Hospital, Region Värmland, Karlstad, Sweden., Kjaer A; Department of Clinical Physiology and Nuclear Medicine & Cluster for Molecular Imaging, Copenhagen University Hospital - Rigshospitalet & Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark., Petersen RF; Department of Bacteria, Fungi and Parasites, Statens Serum Institut, Copenhagen, Denmark., Ostrowski SR; Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Harritshøj L; Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Jacobsen S; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.; Copenhagen Research Center for Autoimmune Connective Tissue Diseases - COPEACT, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Overgaard U; Department of Hematology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Krogfelt KA; Department of Science and Environment, Roskilde University, Universitetsvej 1, 4000, Roskilde, Denmark., Lebech AM; Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Blegdamsvej 9, 2100, Copenhagen, Denmark.; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Mens H; Department of Infectious Diseases, Copenhagen University Hospital, Rigshospitalet, Blegdamsvej 9, 2100, Copenhagen, Denmark.
Jazyk: angličtina
Zdroj: Annals of clinical microbiology and antimicrobials [Ann Clin Microbiol Antimicrob] 2023 Mar 20; Vol. 22 (1), pp. 20. Date of Electronic Publication: 2023 Mar 20.
DOI: 10.1186/s12941-023-00571-5
Abstrakt: Background: The tick-borne bacterium, Neoehrlichia mikurensis (N. mikurensis) can cause severe febrile illness and thromboembolic complications in immunocompromised individuals. We investigated the presence of N. mikurensis DNA in retrospectively collected plasma from a well-characterized cohort of Danish immunocompromised patients.
Methods: Plasma samples from 239 patients with immune dysfunction related to hematological or rheumatological disease or due to immunosuppressive therapy, were retrieved from a transdisciplinary biobank (PERSIMUNE) at Rigshospitalet, Copenhagen, Denmark. Serving as immunocompetent controls, plasma samples from 192 blood donors were included. All samples were collected between 2015 and 2019. Real-time PCR targeting the groEL gene was used to detect N. mikurensis DNA. Sequencing was used for confirmation. Borrelia burgdorferi sensu lato IgG antibodies were detected by ELISA as a proxy of tick exposure. Prevalence was compared using Fisher's exact test.
Results: Neoehrlichia mikurensis DNA was detected in 3/239 (1.3%, 95% confidence interval (CI): 0.3 - 3.6%) patients, all of whom primarily had a hematological disease. Follow-up samples of these patients were negative. N. mikurensis DNA was not detected in any of the blood donor samples. IgG antibodies against B. burgdorferi s.l. were detected with similar prevalence in immunocompromised patients and blood donors, i.e., 18/239 (7.5%, 95% CI: 4.8-11.5%) and 11/192 (5.7%, 95%: CI 3.2-10.0%).
Conclusion: In this study, patients with N. mikurensis were not identified by clinical indication and N. mikurensis may therefore be underdiagnosed in Danish patients. Further investigations are needed to explore the clinical significance and implications of this infection.
(© 2023. The Author(s).)
Databáze: MEDLINE
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