Evolutionary conservation of the fidelity of transcription.

Autor: Chung C; School of Gerontology, University of Southern California, Los Angeles, CA, USA., Verheijen BM; School of Gerontology, University of Southern California, Los Angeles, CA, USA., Navapanich Z; School of Gerontology, University of Southern California, Los Angeles, CA, USA., McGann EG; School of Gerontology, University of Southern California, Los Angeles, CA, USA., Shemtov S; School of Gerontology, University of Southern California, Los Angeles, CA, USA., Lai GJ; School of Gerontology, University of Southern California, Los Angeles, CA, USA., Arora P; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA., Towheed A; Children's hospital of Philadelphia, Center for Mitochondrial and Epigenomic Medicine, Philadelphia, PA, USA., Haroon S; Children's hospital of Philadelphia, Center for Mitochondrial and Epigenomic Medicine, Philadelphia, PA, USA., Holczbauer A; Children's hospital of Philadelphia, Center for Mitochondrial and Epigenomic Medicine, Philadelphia, PA, USA., Chang S; Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Manojlovic Z; Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Simpson S; College of Life Sciences and Agriculture, University of New Hampshire, Durham, NH, USA., Thomas KW; College of Life Sciences and Agriculture, University of New Hampshire, Durham, NH, USA., Kaplan C; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA., van Hasselt P; Department of Metabolic Disease, University of Utrecht, Utrecht, the Netherlands., Timmers M; Department of Urology, Medical Center - University of Freiburg, Freiburg, Germany.; German Cancer Consortium (DKTK) Partner Site Freiburg, German Cancer Research Center (DKFZ), Heidelberg, Germany., Erie D; Department of Chemistry, University of North Carolina, Chapel Hill, NC, USA., Chen L; Department of Molecular and Cellular Biology, University of Southern California, Los Angeles, CA, USA., Gout JF; Department of Biological Sciences, Mississippi State University, Mississippi State, MS, USA., Vermulst M; School of Gerontology, University of Southern California, Los Angeles, CA, USA. vermulst@usc.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Mar 20; Vol. 14 (1), pp. 1547. Date of Electronic Publication: 2023 Mar 20.
DOI: 10.1038/s41467-023-36525-w
Abstrakt: Accurate transcription is required for the faithful expression of genetic information. However, relatively little is known about the molecular mechanisms that control the fidelity of transcription, or the conservation of these mechanisms across the tree of life. To address these issues, we measured the error rate of transcription in five organisms of increasing complexity and found that the error rate of RNA polymerase II ranges from 2.9 × 10 -6 ± 1.9 × 10 -7 /bp in yeast to 4.0 × 10 -6 ± 5.2 × 10 -7 /bp in worms, 5.69 × 10 -6 ± 8.2 × 10 -7 /bp in flies, 4.9 × 10 -6 ± 3.6 × 10 -7 /bp in mouse cells and 4.7 × 10 -6 ± 9.9 × 10 -8 /bp in human cells. These error rates were modified by various factors including aging, mutagen treatment and gene modifications. For example, the deletion or modification of several related genes increased the error rate substantially in both yeast and human cells. This research highlights the evolutionary conservation of factors that control the fidelity of transcription. Additionally, these experiments provide a reasonable estimate of the error rate of transcription in human cells and identify disease alleles in a subunit of RNA polymerase II that display error-prone transcription. Finally, we provide evidence suggesting that the error rate and spectrum of transcription co-evolved with our genetic code.
(© 2023. The Author(s).)
Databáze: MEDLINE
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