Characterization of atypical Mycoplasma anserisalpingitidis strains.

Autor: Kovács ÁB; Veterinary Medical Research Institute, Budapest, Hungary; National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Veterinary Medical Research Institute, Budapest, Hungary., Wehmann E; Veterinary Medical Research Institute, Budapest, Hungary; National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Veterinary Medical Research Institute, Budapest, Hungary., Grózner D; Veterinary Medical Research Institute, Budapest, Hungary., Bali K; Veterinary Medical Research Institute, Budapest, Hungary., Nemesházi E; Veterinary Medical Research Institute, Budapest, Hungary., Hrivnák V; Veterinary Medical Research Institute, Budapest, Hungary., Morrow CJ; Asia Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Victoria, Australia; Bioproperties Pty Ltd., Victoria, Australia., Bányai K; Veterinary Medical Research Institute, Budapest, Hungary; National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Veterinary Medical Research Institute, Budapest, Hungary; Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary., Kreizinger Z; Veterinary Medical Research Institute, Budapest, Hungary; MolliScience Kft., Biatorbágy, Hungary., Gyuranecz M; Veterinary Medical Research Institute, Budapest, Hungary; National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Veterinary Medical Research Institute, Budapest, Hungary; MolliScience Kft., Biatorbágy, Hungary. Electronic address: m.gyuranecz@gmail.com.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2023 May; Vol. 280, pp. 109722. Date of Electronic Publication: 2023 Mar 11.
DOI: 10.1016/j.vetmic.2023.109722
Abstrakt: Mycoplasma anserisalpingitidis is a waterfowl colonizing mycoplasma, mainly found in geese. In this study, we compared the whole genomes of five atypical M. anserisalpingitidis strains originating from China, Vietnam and Hungary, with the rest of the collection. Common methods used in the description of species are genomic analyses like the analysis of 16 S - intergenic transcribed spacer (ITS) - 23 S rRNA, of housekeeping genes, of the average nucleotide identity (ANI) and average amino acid identity (AAI) and phenotypic analyses like testing the growth inhibition and the growth parameters of the strains. The atypical strains showed notable genomic differences in all of the genetic analyses: on average ANI and AAI 95% (M. anserisalpingitidis ANI Minimum: 92.45, Maximum: 95.10; AAI Minimum: 93.34, Maximum: 96.37). The atypical strains formed a separate branch among the M. anserisalpingitidis strains in all phylogenetic studies. The small genome size and possibly higher mutation rate of the M. anserisalpingitidis species likely contributed to the observed genetic difference. Based on genetic analyses, the studied strains clearly represent a new genotype of M. anserisalpingitidis. The atypical strains showed slower growth in the medium containing fructose and three of the atypical strains showed diminished growth in the inhibition test. However, no definitive geno-phenotype associations were found regarding the fructose metabolism pathway in the atypical strains. The atypical strains are potentially at an early stage of speciation.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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