| Autor: |
Kim JH; Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99 Daehak-ro, Yuseong, Daejeon 34134, Republic of Korea., Ham YJ; Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99 Daehak-ro, Yuseong, Daejeon 34134, Republic of Korea., Kim SB; Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99 Daehak-ro, Yuseong, Daejeon 34134, Republic of Korea. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2023 Mar; Vol. 73 (3). |
| DOI: |
10.1099/ijsem.0.005755 |
| Abstrakt: |
An actinobacterial strain designated MMS20-HV4-12 T , displaying a high hydrolytic potential for various substrates, was isolated from a riverside soil sample and characterized by polyphasic taxonomic analysis. Growth occurred at 10-37 °C (optimum, 30°C), with NaCl concentrations of 0-4 % (optimum, 0 %) and at pH 7-9 (optimum, pH 8). MMS20-HV4-12 T was catalase-positive, oxidase-negative, rod-shaped and formed creamy white-coloured colonies. Based on the results of 16S rRNA gene sequence analysis, MMS20-HV4-12 T was found to be mostly related to the type strains of Nocardioides alpinus (98.3 % sequence similarity), Nocardioides furvisabuli (98.1 %) and Nocardioides zeicaulis (98.0 %). MMS20-HV4-12 T showed optimal growth on Reaoner's 2A agar, forming white-coloured colonies. The diagnostic polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, the major fatty acids were iso-C 16 : 0 , C 17 : 1 ω8 c and 10-methyl-C 17 : 0 , the major isoprenoid quinone was MK-8(H 4 ), the diagnostic cell-wall sugar was galactose, and the cell-wall diamino acid was ll-diaminopimelic acid. The genome of MMS20-HV4-12 T was 4.47 Mbp in size with a G+C content of 72.9 mol%. The genome based analysis indicated low relatedness between MMS20-HV4-12 T and all compared species of Nocardioides , as the highest digital DNA-DNA hybridization and the orthologous average nucleotide identity values were 26.8 and 83.8% respectively. Based on genotypic, phenotypic and phylogenomic characterization, MMS20-HV4-12 T evidently represents a novel species of genus Nocardioides , for which the name Nocardioides okcheonensis sp. nov. (type strain=MMS20-HV4-12 T =KCTC 49651 T =LMG 32360 T ) is proposed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
