Characterization of hepatic fatty acids using magnetic resonance spectroscopy for the assessment of treatment response to metformin in an eNOS -/- mouse model of metabolic nonalcoholic fatty liver disease/nonalcoholic steatohepatitis.

Autor: Lavin B; School of Biomedical Engineering Imaging Sciences, King's College London, London, UK.; BHF Centre of Research Excellence, Cardiovascular Division, King's College London, London, UK.; Department of Biochemistry and Molecular Biology, School of Chemistry, Complutense University, Madrid, Spain., Eykyn TR; School of Biomedical Engineering Imaging Sciences, King's College London, London, UK.; BHF Centre of Research Excellence, Cardiovascular Division, King's College London, London, UK., Phinikaridou A; School of Biomedical Engineering Imaging Sciences, King's College London, London, UK.; BHF Centre of Research Excellence, Cardiovascular Division, King's College London, London, UK., Xavier A; Biomedical Engineering, Faculty of Engineering, Universidad de Santiago de Chile, Santiago, Chile.; ANID - Millennium Science Initiative Program - Millennium Institute Intelligent Healthcare Engineering, Santiago, Chile., Kumar S; School of Biomedical Engineering Imaging Sciences, King's College London, London, UK., Buqué X; Physiology Department, School of Medicine and Nursing, Universidad del País Vasco UPV/EHU, Vizcaya, Spain.; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain., Aspichueta P; Physiology Department, School of Medicine and Nursing, Universidad del País Vasco UPV/EHU, Vizcaya, Spain.; Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.; CIBER de enfermedades hepáticas y digestivas (CIBERehd), Spain., Sing-Long C; ANID - Millennium Science Initiative Program - Millennium Institute Intelligent Healthcare Engineering, Santiago, Chile.; School of Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile., Arrese M; ANID - Millennium Science Initiative Program - Millennium Institute Intelligent Healthcare Engineering, Santiago, Chile.; Gastroenterology Department, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile., Botnar RM; School of Biomedical Engineering Imaging Sciences, King's College London, London, UK.; BHF Centre of Research Excellence, Cardiovascular Division, King's College London, London, UK.; ANID - Millennium Science Initiative Program - Millennium Institute Intelligent Healthcare Engineering, Santiago, Chile.; School of Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile., Andia ME; ANID - Millennium Science Initiative Program - Millennium Institute Intelligent Healthcare Engineering, Santiago, Chile.; School of Medicine and Centro de Envejecimiento y Regeneración (CARE), Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
Jazyk: angličtina
Zdroj: NMR in biomedicine [NMR Biomed] 2023 Aug; Vol. 36 (8), pp. e4932. Date of Electronic Publication: 2023 Apr 11.
DOI: 10.1002/nbm.4932
Abstrakt: Nonalcoholic fatty liver disease (NAFLD) is the leading cause of chronic liver disease worldwide. Liver biopsy remains the gold standard for diagnosis and staging of disease. There is a clinical need for noninvasive diagnostic tools for risk stratification, follow-up, and monitoring treatment response that are currently lacking, as well as preclinical models that recapitulate the etiology of the human condition. We have characterized the progression of NAFLD in eNOS -/- mice fed a high fat diet (HFD) using noninvasive Dixon-based magnetic resonance imaging and single voxel STEAM spectroscopy-based protocols to measure liver fat fraction at 3 T. After 8 weeks of diet intervention, eNOS -/- mice exhibited significant accumulation of intra-abdominal and liver fat compared with control mice. Liver fat fraction measured by 1 H-MRS in vivo showed a good correlation with the NAFLD activity score measured by histology. Treatment of HFD-fed NOS3 -/- mice with metformin showed significantly reduced liver fat fraction and altered hepatic lipidomic profile compared with untreated mice. Our results show the potential of in vivo liver MRI and 1 H-MRS to noninvasively diagnose and stage the progression of NAFLD and to monitor treatment response in an eNOS -/- murine model that represents the classic NAFLD phenotype associated with metabolic syndrome.
(© 2023 The Authors. NMR in Biomedicine published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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