Sweroside: An iridoid glycoside of potential neuroprotective, antidiabetic, and antioxidant activities supported by molecular docking.

Autor: Zengin G; Department of Biology, Faculty of Science, Selcuk University, Konya, 42130, Turkey. gokhanzengin@selcuk.edu.tr., El-Raey M; Department of Phytochemistry and Plant Systematics, National Research Centre, Dokki, 12622, Cairo, Egypt., El-Kashak W; Department of Chemistry of Natural Compounds, National Research Centre, Dokki, 12622, Cairo, Egypt., Batiha GE; Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour, 22511, AlBeheira, Egypt., Althumairy D; Department of Biological Sciences, King Faisal University, Al-Ahsa, Al-Hofuf, 31982, Saudi Arabia., Alamer S; Department of Biological Sciences, King Faisal University, Al-Ahsa, Al-Hofuf, 31982, Saudi Arabia., Mostafa NM; Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, 11566, Cairo, Egypt., Eldahshan OA; Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, 11566, Cairo, Egypt.; Center of Drug Discovery Research and Development, Ain Shams University, Abbassia, 11566, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Amino acids [Amino Acids] 2023 Dec; Vol. 55 (12), pp. 1765-1774. Date of Electronic Publication: 2023 Mar 20.
DOI: 10.1007/s00726-023-03262-9
Abstrakt: Oxidative stress can be a series burden on human health and may lead to many chronic diseases such as diabetes and neurological disorders. The use of natural products to scavenge the reactive oxygen species has attracted the attention of many researchers, to safely manage these conditions with fewer side effects, in available and cost-effective ways. The current study aimed at the isolation and structure elucidation of sweroside from Schenkia spicata (Gentianaceae) and the evaluation of its antioxidant, antidiabetic, neuroprotective, and enzyme inhibitory potential via in vitro and in silico studies. The antioxidant potential was evaluated by a variety of assays as ABTS, CUPRAC and FRAP, showing values of 0.34 ± 0.08, 21.14 ± 0.43, and 12.32 ± 0.20 mg TE/g, respectively, while demonstrating 0.75 ± 0.03 mmol TE/g for phosphomolybdenum (PBD) assay. Acetylcholinestrase (AChE), butyrylcholinesterase (BChE) and tyrosinase inhibitory activities were used to evaluate the neuroprotective effect, while the antidiabetic potential was evaluated by measuring α-amylase and glucosidase inhibitory activities. Results revealed that sweroside showed antioxidant and inhibitory effects on the enzymes tested with the exception of AChE. It demonstrated good tyrosinase inhibitory ability with 55.06 ± 1.85 mg Kojic acid equivalent /g. Regarding the antidiabetic ability, the compound displayed both amylase and glucosidase (0.10 ± 0.01 and 1.54 ± 0.01 mmol Acarbose equivalent/g, respectively) inhibitory activities. Molecular docking studies of sweroside on the active sites of the aforementioned enzymes in addition to NADPH oxidase were performed using Discovery Studio 4.1 software. Results revealed good binding affinities of sweroside to these enzymes mainly through hydrogen bonds and van der Waals interactions. Sweroside can be an important antioxidant and enzyme inhibitory supplement, yet further in vivo and clinical studies are required.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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