In vitro effects of tannin and extracts of Bridelia ferruginea and Mitragyna inermis on the exsheathment of infective larvae of Haemonchuscontortus .

Autor:	Tchetan E; Laboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, Cotonou, Bénin.; Laboratoire de Biotechnologie et d'Amélioration Animale, Faculté des Sciences Agronomiques, Institut des Sciences Biomédicales Appliquées, Université d'Abomey-Calavi, Cotonou, Bénin., Azando EVB; Laboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, Cotonou, Bénin.; Laboratoire de Biotechnologie et d'Amélioration Animale, Faculté des Sciences Agronomiques, Institut des Sciences Biomédicales Appliquées, Université d'Abomey-Calavi, Cotonou, Bénin.; Laboratoire d'Ecologie, de Santé et de Productions Animales, Département des Sciences et Techniques de Production Animale et Halieutique, Faculté d'Agronomie, Université de Parakou, Parakou, Bénin., Olounladé PA; Laboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, Cotonou, Bénin.; Laboratoire de Biotechnologie et d'Amélioration Animale, Faculté des Sciences Agronomiques, Institut des Sciences Biomédicales Appliquées, Université d'Abomey-Calavi, Cotonou, Bénin.; Laboratoire des Sciences Animale et Halieutique (Lasah), Unité de Recherches Zootechniques et Système d'Elevage, Ecole de Gestion et d'Exploitation des Systèmes d'Elevage, Université Nationale d'Agriculture, Porto Novo, Bénin., Alowanou GG; Laboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, Cotonou, Bénin.; Département des Sciences et Techniques Agricoles, Ecole Normale Supérieure de l'Enseignement Technique, Université Nationale des Sciences, Technologies, Ingénierie et Mathématiques, Abomey, Bénin., Hounzangbé-Adoté SM; Laboratoire d'Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d'Abomey Calavi, Cotonou, Bénin.
Jazyk:	angličtina
Zdroj:	International journal of veterinary science and medicine [Int J Vet Sci Med] 2020 Nov 23; Vol. 8 (1), pp. 93-99. Date of Electronic Publication: 2020 Nov 23 (Print Publication: 2020).
DOI:	10.1080/23144599.2020.1837056
Abstrakt:	Bridelia ferruginea (Euphorbiaceae) and Mitragyna inermis (Rubiaceae) are two plants of the beninese pharmacopeia used in vivo for the control of gastrointestinal nematodes (GINs) in small ruminants. The objective of the present study is to explore the mechanism of bioactive compounds involved in the action of these two plants on the third-stage infective larvae (L3s) of Haemonchus contortus . Thus, sheathed L3s of H. contortus were incubated with acetone extracts of B. ferruginea and M. inermis at concentrations of 0, 150, 300, 600 and 1200 µg/mL for 3 h at 25°C. The L3s were then washed and artificially submitted to exsheathment in the presence of sodium hypochlorite solution. The role of tannins was verified by adding a tannin inhibitor, polyvinyl polypyrrolidone (PVPP), to the acetone extracts of these two plants for 2 h at 25°C. Acetone extracts from B. ferruginea and M. inermis inhibited the exsheathment of H. contortus larvae ( p < 0.001) and this inhibitory effect was dose-dependent for M. inermis at the concentrations tested. Treatment of B. ferruginea and M. inermis extracts with PVPP was associated with a partial restoration of the exsheathment kinetics of H. contortus larvae ( p < 0.001), confirming the predominant role of tannins but also the residual role of other secondary metabolites. These in vitro results suggest that these plants are endowed with anthelmintic (AHs) properties and therefore likely to be used as alternatives to synthetic molecules. Competing Interests: The authors declare that they have no competing interests. (© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)
Databáze:	MEDLINE
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